Chryseobacterium endalhagicum sp. nov., isolated from seed of leguminous plant

A Gram-stain-negative, yellow-pigmented bacterium, designated as L7^T, was isolated from seeds of Alhagi sparsifolia Shap., a leguminous plant that grows in northwest PR China. Strain L7^T was found to be non-flagellated, non-spore forming rods which can grow at 10-37 °C, pH 6.0-8.5 and in 0-3 % (v/w) NaCl concentration. The 16S rRNA gene sequence analysis showed that strain L7^T belongs to the genus Chryseobacterium with sequence similarities to Chryseobacterium vietnamense GIMN1.005^T (98.1%), C. bernardetii NCCTC13530^T (98.0%), C. vrystaatense LMG 22846^T (97.9%), C. nakagawai NCTC13529^T (97.7%), C. shigense DSM 17126^T (97.6%) and C. rhizosphaerae RSB3-1^T (97.5%). The average nucleotide identity of strain L7^T to 31 reference strains were 78.6-85.6 %, lower than the species delineation threshold of 95 %. MK-6 was the only respiratory quinone of L7^T and major fatty acids were iso-C_15 : 0, iso-C_17 : 0 3-OH, C_16 : 1  ω7c and/or C_16 : 1  ω6c, isoC_17 : 1  ω9c and/or C_16 : 0 10-methyl. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, three unidentified aminophospholipids, two unidentified aminolipids, three unidentified glycolipids and two unidentified lipids. The G+C content of the genome was 38.58 mol%. On the basis of polyphasic taxonomy analyses in this study, strain L7^T is considered to represent a novel species in the genus Chryseobacterium, for which the name Chryseobacterium endalhagicum sp. nov. is proposed. The type strain is L7^T (=MCCC 1K05687^T=JCM 34506^T).