Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Linear ubiquitination triggers Amph-mediated T-tubule biogenesis.

Science advances·2026
Same author

Towards global healthy longevity: report from the 1st World Longevity Summit in Kyotango, Japan.

npj aging·2025
Same author

Efficacy and Safety of Edoxaban in Anticoagulant Therapy Early After Surgical Bioprosthetic Valve Replacement: A Randomized Clinical Trial.

Circulation. Cardiovascular interventions·2025
Same author

Trends of aortic valve-preserving surgery in japan: an updated five-year nationwide survey.

General thoracic and cardiovascular surgery·2025
Same author

Sephin1 suppresses ER stress-induced cell death by inhibiting the formation of PP2A holoenzyme.

Cell death & disease·2025
Same author

Use of a mouse-human chimeric anti-α-galactosidase A monoclonal antibody as a reference for measuring serum antidrug antibody titers in patients with Fabry disease.

Molecular genetics and metabolism·2024

Related Experiment Video

Updated: Oct 14, 2025

Engineered Vascularized Muscle Flap
08:18

Engineered Vascularized Muscle Flap

Published on: January 11, 2016

8.4K

A tissue-engineered, decellularized, connective tissue membrane for allogeneic arterial patch implantation.

Masashi Yamanami1, Keiichi Kanda1, Kazuki Morimoto1

  • 1Department of Cardiovascular Surgery, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan.

Artificial Organs
|November 5, 2021
PubMed
Summary

Decellularized connective tissue membranes from donor dogs can be stored and used as allogeneic cardiovascular grafts. This method shows potential for creating vascular patches, even in hosts with limited regenerative capacity.

Keywords:
allogeneic arterial patch implantationconnective tissuedecellularizationtissue engineering

More Related Videos

Generation and Grafting of Tissue-engineered Vessels in a Mouse Model
13:04

Generation and Grafting of Tissue-engineered Vessels in a Mouse Model

Published on: March 18, 2015

12.2K
Transplantation of a 3D Bioprinted Patch in a Murine Model of Myocardial Infarction
07:47

Transplantation of a 3D Bioprinted Patch in a Murine Model of Myocardial Infarction

Published on: September 26, 2020

6.8K

Related Experiment Videos

Last Updated: Oct 14, 2025

Engineered Vascularized Muscle Flap
08:18

Engineered Vascularized Muscle Flap

Published on: January 11, 2016

8.4K
Generation and Grafting of Tissue-engineered Vessels in a Mouse Model
13:04

Generation and Grafting of Tissue-engineered Vessels in a Mouse Model

Published on: March 18, 2015

12.2K
Transplantation of a 3D Bioprinted Patch in a Murine Model of Myocardial Infarction
07:47

Transplantation of a 3D Bioprinted Patch in a Murine Model of Myocardial Infarction

Published on: September 26, 2020

6.8K

Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Vascular Surgery

Background:

  • Previous in vivo tissue-engineered vascular grafts relied on host health, limiting application in patients with suppressed regenerative abilities.
  • Allogeneic grafts from healthy donors are a potential alternative for cardiovascular repair.
  • This study investigated the feasibility of fabricating allogeneic cardiovascular grafts in an animal model.

Purpose of the Study:

  • To evaluate the potential of decellularized connective tissue membranes as allogeneic cardiovascular grafts.
  • To assess the biocompatibility and integration of these grafts in a vascular setting.

Main Methods:

  • Silicone molds were used to create tubular connective tissues in dogs, which were harvested after four weeks.
  • Harvested tissues were decellularized, stored, and fashioned into elliptical sheets for implantation as vascular patches in recipient dogs' carotid arteries.
  • Grafts were histologically evaluated at one, two, and four weeks post-implantation.

Main Results:

  • Echocardiography revealed no aneurysmal changes in the implanted grafts.
  • Histological analysis showed neointima formation and cell infiltration within the graft wall.
  • α-SMA-positive cells were detected in the neointima and graft wall at two and four weeks, with endothelialization observed at four weeks.

Conclusions:

  • Decellularized connective tissue membranes can be successfully prepared and stored for future use.
  • These membranes demonstrate potential as allogeneic cardiovascular grafts for vascular repair applications.