Analyte-restrained silver coating of gold nanostructures: an efficient strategy to advance multicolorimetric probes

Visual detection based on gold nanorods (AuNRs) has gained tremendous attention in sensing applications owing to the potential for simple, inexpensive, instrument-free, and on-site detection. The proper selection of the mechanism involved in the interaction between the analyte and the nanostructure plays a significant role in designing a selective and multicolorimetric probe for visual purposes. A winning mechanism to develop multicolorimetric probes is the silver metalization of AuNRs. Herein, an unprecedented idea is presented to expand the variety of multicolorimetric sensors relying on the mechanism of silver deposition. We introduce the anti-silver deposition mechanism in which the analyte directly or indirectly restrains the silver coating of AuNRs. To ascertain the anti-silver deposition mechanism, we have exploited the proposed idea for the direct detection of nitrate. The presence of nitrate (as restrainer agent), which was firstly treated with ascorbic acid (as reducing agent), induced a decrease in the spectral blueshift of AuNRs along with diverse sharp color transitions from reddish-orange (blank) to maroon, wine, berry/purple, dark blue, teal, green, seafoam, and mint. The difference in the spectrum area of the probe in the absent (S_o) and presence (S) of nitrate were linearly proportional to nitrate concentration in the range of 0.5-5.5 mmol l^-1and the limit of detection was calculated to be 465μmol l^-1. Furthermore, the practicability of the multicolor probe was assessed by the determination of nitrate in complex environmental samples.