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Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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Related Experiment Video

Updated: Oct 14, 2025

Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP

Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP

Published on: September 8, 2023

COVID-19 rapid diagnostics: practice review.

Charles Reynard^1,2, Joy A Allen^3,4, Bethany Shinkins⁵

¹Emergency Department, Manchester University NHS Foundation Trust, Manchester, UK charlie.reynard@nhs.net.

Emergency Medicine Journal : EMJ

|November 6, 2021

Summary

This summary is machine-generated.

Rapid point-of-care tests for SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) aid in quick COVID-19 diagnosis. Understanding diagnostic technologies is crucial for effective patient management and infection control.

Keywords:

COVID-19 diagnosis emergency department infectious diseases

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Last Updated: Oct 14, 2025

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Published on: September 8, 2023

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Published on: February 11, 2022

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Published on: March 31, 2021

Area of Science:

Clinical diagnostics
Infectious disease epidemiology
Medical technology assessment

Background:

Point-of-care tests (POCTs) for SARS-CoV-2 are vital for rapid COVID-19 diagnosis and controlling nosocomial spread.
Increasing co-circulation of SARS-CoV-2 with other respiratory viruses necessitates multiplex diagnostic capabilities.

Purpose of the Study:

To review the relative merits and characteristics of various commercially available SARS-CoV-2 POCT technologies.
To inform clinicians on selecting and utilizing POCTs effectively within healthcare settings.

Main Methods:

Practice review by members of the UK's COVID-19 National Diagnostic Research and Evaluation programme.
Discussion of commercially available SARS-CoV-2 diagnostic technologies, focusing on their performance and test characteristics.

Main Results:

Different POCT technologies possess distinct strengths and weaknesses impacting their clinical utility.
Device performance and diagnostic accuracy are influenced by the deployment context and user experience.

Conclusions:

A thorough understanding of POCT technology characteristics is essential for optimal patient care and healthcare system benefit.
This review provides insights into SARS-CoV-2 POCTs but does not endorse specific products.