Effects of Nano-copper Oxide on Antioxidant Function of Copper-Deficient Kazakh Sheep

Kazakh sheep are vital to the production system of the Barkol prairie. The purpose of this study was to determine the effect of nano-copper oxide (Nano-Cu₂O) on the antioxidant system of Cu-deficient Kazakh sheep in the Barkol prairie in Xinjiang, China. We analyzed mineral contents in soil, forage, and animal tissues. Blood parameters were also measured at the same time. The results showed that compared with healthy grassland, the Cu content in the soil and forage in Cu-deficient pastures was significantly lower than that in healthy grassland (P < 0.01). The Cu content in the blood, wool, and liver of Cu-deficient Kazakh sheep was significantly lower than that of healthy animals (P < 0.01). After Kazakh sheep were supplemented with Nano-Cu₂O or CuSO₄, the blood Cu concentration increased significantly (P < 0.01). From the 5th day, the Cu content of the Nano-Cu₂O group was significantly higher than that of the CuSO₄ group. The levels of hemoglobin (Hb), erythrocyte count (RBC), and packed cell volume (PCV) in the two experimental groups were significantly higher than those in Cu-deficient Kazakh sheep (P < 0.01). Compared with Cu-deficient Kazakh sheep, the serum ceruloplasmin (Cp) level of the two experimental groups increased significantly (P < 0.01), while the serum lactate dehydrogenase (LDH), alkaline phosphatase (AKP), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) decreased significantly (P < 0.01). Compared with Cu-deficient Kazakh sheep, the activities of serum superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT), and total antioxidant capacity (T-AOC) in Nano-Cu₂O and CuSO₄ groups increased significantly (P < 0.01), while the level of serum malondialdehyde (MDA) decreased significantly (P < 0.01). Therefore, Nano-Cu₂O could not only significantly increase the Cu content in the blood of Cu-deficient Kazakh sheep, but also greatly improve the antioxidant capacity.