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Meiosis II

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Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos

Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos

Published on: February 22, 2016

Cell-cycle dependent GATA2 subcellular localization in mouse 2-cell embryos.

Masaya Komatsu¹, Hayato Tsukahara¹, Hanako Bai¹

¹Laboratory of Animal Genetics and Reproduction, Research Faculty of Agriculture, Hokkaido University, Sapporo, 060-8589, Japan.

Biochemical and Biophysical Research Communications

|November 6, 2021

Summary

GATA2 protein localization dynamically changes during the cell cycle in early mouse embryos. Mitotic GATA2 aggregation is crucial for proper cell cycle progression and embryonic development.

Keywords:

2-Cell embryos Cell cycle GATA2 Intracellular localization Mouse

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Published on: February 24, 2017

Area of Science:

Developmental Biology
Molecular Biology
Cell Biology

Background:

GATA factors are crucial transcription factors regulating gene expression during embryonic development.
Early embryonic development involves significant gene expression changes, particularly at the 2-cell stage.

Purpose of the Study:

To investigate the dynamic localization of GATA2 protein in mouse embryos at the 2-cell stage.
To understand the relationship between GATA2 protein localization and cell cycle progression.

Main Methods:

Immunofluorescence analysis of GATA2 protein localization in 2-cell mouse embryos during interphase and mitosis.
Overexpression of GFP-labeled GATA2 protein in 2-cell embryos to assess effects on cell cycle progression.

Main Results:

During interphase, GATA2 localized to the nucleus.
During mitosis, GATA2 formed focally-aggregated spots around the nucleus.
GATA2 overexpression arrested mitosis and prevented focal aggregation, without affecting murine endogenous retrovirus-L expression.

Conclusions:

GATA2 protein localization is cell cycle-dependent in 2-cell mouse embryos.
Focal GATA2 aggregation during mitosis is essential for correct cell cycle progression.