Microplastics reduce the bioaccumulation and oxidative stress damage of triazole fungicides in fish

Microplastics (MPs) and pesticides are typical representatives of harmful chemicals in polluted waters. It is understood that the combined toxicity may differ from that of a single toxic substance. Although their combined toxicities on aquatic organisms have practical significance and research value, they have received little attention due to their complicated interaction, and the mechanism has rarely been reported. In this paper, we designed a study to investigate the single and combined effects of polystyrene microplastics (PS-MPs) and the triazole fungicide difenoconazole on zebrafish, and to explore the mechanism of this effect. The results showed that PS-MPs could reduce the bioaccumulation of difenoconazole in zebrafish to a certain extent and alleviate the oxidative stress damage of difenoconazole in the zebrafish liver. The transcriptome and qRT-PCR data revealed the association of multiple pathways in the difenoconazole response, while the presence of PS-MPs ameliorated this effect in gene expression changes. Due to the properties of PS-MPs and the interaction between them, the toxic effect of difenoconazole when combined with PS-MPs is more prominent. These results provide a novel aspect to understand the environmental behavior of MPs and to evaluate the combined effect of MPs and pesticides on aquatic food.