Related Experiment Video

Updated: Oct 14, 2025

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
10:27

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

Published on: October 21, 2022

1.7K

Circular RNA circ_GLIS2 suppresses hepatocellular carcinoma growth and metastasis

Wu Xing1, Peng-Cheng Zhou2,3, Hao-Ye Zhang2

  • 1Department of Radiology, Xiangya Hospital, Central South University, Changsha, China.

Liver International : Official Journal of the International Association for the Study of the Liver
|November 7, 2021
PubMed
Abstract

Insights

Circular RNA circ_GLIS2 suppresses hepatocellular carcinoma (HCC) growth and metastasis. Low circ_GLIS2 levels correlate with poor patient survival, suggesting its potential as a therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Regulation

Background:

  • Hepatocellular carcinoma (HCC) is a major cause of cancer-related mortality worldwide.
  • Understanding the molecular mechanisms underlying HCC progression is crucial for developing effective treatments.

Purpose of the Study:

  • To investigate the functional role of circ_GLIS2 in the molecular mechanisms of HCC.
  • To explore the potential of circ_GLIS2 as a diagnostic marker or therapeutic target for HCC.

Main Methods:

  • Analysis of circ_GLIS2 and GLIS2 mRNA levels in HCC tissues and cell lines.
  • Functional assays (proliferation, apoptosis, migration, invasion) to assess the impact of circ_GLIS2.
  • In vivo studies using a nude mouse xenograft model to evaluate tumor growth and metastasis.

Main Results:

  • Circ_GLIS2 and GLIS2 mRNA were downregulated in HCC, correlating with poor patient survival.
  • Overexpression of circ_GLIS2 inhibited HCC cell proliferation, migration, and invasion, while promoting apoptosis.
  • Circ_GLIS2 overexpression suppressed tumor growth and metastasis in vivo.

Conclusions:

  • Circ_GLIS2 acts as a tumor suppressor in HCC by inhibiting proliferation, migration, and invasion, and promoting apoptosis.
  • Circ_GLIS2 represents a promising diagnostic marker and therapeutic target for hepatocellular carcinoma.
Keywords:
EIF4A3circ_GLIS2/GLIS2hepatocellular carcinomamethylation

More Related Videos

Use of Alu Element Containing Minigenes to Analyze Circular RNAs13:10

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Published on: March 10, 2020

7.5K
Identification of Circular RNAs using RNA Sequencing08:25

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

12.4K

Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.4K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.2K
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.7K
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
17.2K
The Retinoblastoma Gene01:20

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.3K
Rous Sarcoma Virus (RSV) and Cancer01:03

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...
5.6K