Related Experiment Video
Updated: Jul 10, 2026
Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms
Published on: April 17, 2017
A competitive ligand-binding assay to detect neutralizing antibodies to a bispecific drug using a multiplex Meso
Vitaly Ablamunits1, Soma Basak1, Rosemary Lawrence-Henderson1
1Biomedicine Design, Pfizer, Inc. 1 Burtt Rd, Building G, Andover, MA 01810, USA.
Abstract:
Background: Monitoring appearance of neutralizing antibodies (NAbs) to multidomain large molecule drugs is a challenging task. Materials & methods: Here, we report development of a competitive ligand-binding assay for detection of NAbs to a bispecific candidate drug using a multiplex Meso Scale Discovery platform, which allows for detection of NAbs to both drug arms in the same sample. Results: The assay has sensitivity better than 250 ng/ml and is tolerant to the presence of drug at concentration >600 μg/ml and to the level of soluble target(s) >400 ng/ml. Conclusion: Our data suggest that multiplex approach can be successfully used for development of NAb assays in competitive ligand-binding assay format.
