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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR and crRNAs02:53

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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CRISPR01:59

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Updated: Oct 14, 2025

Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera H&#252;bner
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CRISPR/Cas9 in lepidopteran insects: Progress, application and prospects.

Jiang-Jie Li1, Yan Shi1, Ji-Nan Wu2

  • 1Key Lab of Integrated Crop Pest Management of Shandong Province, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao, Shandong 266109, PR China; Laboratory of Agrozoology, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, 9000 Ghent, Belgium.

Journal of Insect Physiology
|November 8, 2021
PubMed
Summary

CRISPR gene editing offers precise control for studying lepidopteran insects. This technology advances research in insect development, pest control, and silkworm breeding innovation.

Keywords:
CRISPR/Cas9 systemLepidopteraProspectsResearch progress

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Area of Science:

  • Molecular Biology
  • Genetics
  • Entomology

Background:

  • CRISPR-Cas systems, derived from prokaryotic immune systems, provide robust, specific, and programmable genome editing capabilities.
  • Recent advancements have enabled CRISPR applications across diverse insect sciences, particularly in lepidopterans.

Purpose of the Study:

  • To provide a comprehensive review of CRISPR technology's progress and applications in lepidopteran insects.
  • To highlight the system's principles and its utility in exploring gene functions and developing pest control strategies.

Main Methods:

  • Review of existing literature on CRISPR/Cas applications in lepidopteran insects.
  • Analysis of CRISPR's impact on understanding gene function and its potential for insect modification.

Main Results:

  • CRISPR/Cas has been successfully applied to various lepidopteran species, facilitating gene function studies.
  • The technology shows promise for applications in wing development, pigmentation, reproduction, and pest resistance.

Conclusions:

  • CRISPR/Cas systems are revolutionizing lepidopteran research, offering powerful tools for genetic manipulation.
  • Future prospects include enhanced pest control, improved silkworm breeding, and deeper insights into insect biology.