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Updated: Oct 14, 2025

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
Published on: March 8, 2018
The complete chloroplast genome of Crataegus bretschneideri Schneid. (Rosaceae)
Shuqi Zheng1,2, Han Song3, Ningguang Dong1,2
1Beijing Academy of Forestry and Pomology Sciences, Beijing Academy of Agriculture and Forestry Sciences, Beijing, P.R. China.
Abstract:
Crataegus bretschneideri Schneid., with an unclear phylogenic position, is mainly distributed in northeast and inner mongolia area of China. In this study, the complete chloroplast genome sequence of C. bretschneideri was determined by using Illumina high-throughput sequencing method. The chloroplast genome was 159,607 bp in length and consisted of a large single-copy (LSC) region (87,601 bp), a small single-copy (SSC) region (19,312 bp), separated by a pair of inverted repeat (IRs: 26,347 bp, each) regions. It comprised a total of 114 unique genes, including 80 protein-coding genes, 30 tRNA genes, and 4 rRNA genes. Phylogenetic analysis based on complete chloroplast genomes indicated that C. bretschneideri was closely related to C. marshallii Eggl in the subfamily Maloideae. This complete chloroplast genome will provide valuable insight into evolution, molecular breeding, and phylogenetic analysis of Crataegus species.
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