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Synchronous oscillations in microtubule polymerization.

M F Carlier, R Melki, D Pantaloni

    Proceedings of the National Academy of Sciences of the United States of America
    |August 1, 1987
    PubMed
    Summary

    Microtubule assembly in vitro shows oscillations before steady state under specific conditions. These dynamic microtubule length changes can be simulated by synchronous growth and depolymerization phases.

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    Area of Science:

    • Biochemistry
    • Cell Biology
    • Biophysics

    Background:

    • Microtubule dynamics are crucial for cellular processes.
    • Understanding microtubule assembly kinetics is essential for cell biology research.
    • Previous models did not fully capture the complex dynamics observed in vitro.

    Purpose of the Study:

    • To investigate the oscillatory regime during in vitro microtubule assembly.
    • To characterize the amplitude and duration of these oscillations.
    • To explore the underlying mechanisms driving the observed kinetics.

    Main Methods:

    • In vitro microtubule assembly assays.
    • Turbidity measurements to monitor polymerization.
    • Analysis of microtubule length redistribution.

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  • Kinetic modeling of microtubule dynamics.

    • Main Results:

    • Fast microtubule assembly conditions (high Mg2+, tubulin, no glycerol) induce oscillations.
    • Oscillations reached >50% of maximum turbidity change and persisted for ~20 periods (80s each).
    • Extensive microtubule length redistribution accompanied the oscillations.

    Conclusions:

    • Microtubule assembly can exhibit a distinct oscillatory phase before reaching steady state.
    • Synchronous transitions between growing and depolymerizing phases, limited by nucleotide exchange, likely drive these oscillations.
    • The findings provide insights into the complex regulatory mechanisms of microtubule dynamics.