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A new alkaloid from Portulaca oleracea L. and its anti-inflammatory activity
Fangxuan Liu1, Xinyu Cui2, Yang Duan2
1First Affiliated Hospital of Dalian Medical University, Dalian, P.R. China.
Abstract:
A new alkaloid, identified as (E)-2-(4-hydroxyphenyl)-3-(pyridazin-3-yl)-acrylaldehyde, named oleradazine, was isolated from Portulaca oleracea L., and the structure was elucidated using spectroscopic methods, including 1D and 2D NMR spectroscopic and UHPLC-ESI-QTOF/MS methods. In addition, the compound was used to investigate its anti-inflammatory activity in lipopolysaccharide-stimulated macrophages. It was suggested that the oleradazine can significantly inhibit the inflammatory factors, interleukin 1β and nitric oxide in lipopolysaccharide-stimulated RAW 264.7 cells by enzyme-linked immunosorbent assays.
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