Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

3D point cloud driven organ semantic segmentation to assess maize structural responses along the planting-density gradient.

Plant phenomics (Washington, D.C.)·2026
Same author

Corrigendum to "Associations between multiple metals exposure and cognitive function in the middle-aged and older adults from China: A cross-sectional study" [Environ. Res., 263(Pt 1), 2024, 120038].

Environmental research·2026
Same author

A Self-Immunoregulatory Nanosensitizer for Sonodynamic Cancer Therapy.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Ontology-driven dual-channel relational graph convolutional network (DCR-GCN) for lettuce leaf phenotype classification.

Plant methods·2026
Same author

Microbiomics and metabolomics reveal microbial-metabolic signatures associated with body weight variation in chickens.

Poultry science·2026
Same author

MARCHF6 orchestrates hepatic lipid homeostasis by targeting SREBP1 for ER-associated degradation.

Journal of hepatology·2026

Related Experiment Video

Updated: Oct 14, 2025

An Improved Time- and Labor- Efficient Protocol for Mouse Primary Hepatocyte Isolation
05:42

An Improved Time- and Labor- Efficient Protocol for Mouse Primary Hepatocyte Isolation

Published on: October 25, 2021

10.8K

An Improved Time- and Labor- Efficient Protocol for Mouse Primary Hepatocyte Isolation.

Mingxiao Feng1, Sara Divall2, Sheng Wu3

  • 1Department of Pediatrics, Johns Hopkins University School of Medicine; mfeng8@jhmi.edu.

Journal of Visualized Experiments : Jove
|November 8, 2021
PubMed
Summary
This summary is machine-generated.

This study presents a rapid primary hepatocyte isolation protocol, yielding healthy cells in ~35 minutes for efficient liver in vitro research, particularly glucose metabolism studies.

More Related Videos

Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method
08:04

Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method

Published on: October 23, 2018

19.2K
Isolation of Primary Rat Hepatocytes with Multiparameter Perfusion Control
07:35

Isolation of Primary Rat Hepatocytes with Multiparameter Perfusion Control

Published on: April 5, 2021

5.1K

Related Experiment Videos

Last Updated: Oct 14, 2025

An Improved Time- and Labor- Efficient Protocol for Mouse Primary Hepatocyte Isolation
05:42

An Improved Time- and Labor- Efficient Protocol for Mouse Primary Hepatocyte Isolation

Published on: October 25, 2021

10.8K
Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method
08:04

Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method

Published on: October 23, 2018

19.2K
Isolation of Primary Rat Hepatocytes with Multiparameter Perfusion Control
07:35

Isolation of Primary Rat Hepatocytes with Multiparameter Perfusion Control

Published on: April 5, 2021

5.1K

Area of Science:

  • Hepatology
  • Cell Biology
  • Biochemistry

Background:

  • Primary hepatocytes are crucial for in vitro liver research, especially in glucose metabolism studies.
  • Existing primary hepatocyte isolation protocols are often inefficient due to numerous steps and lengthy reagent preparation.
  • Optimization is needed to improve the efficiency of obtaining healthy primary hepatocytes.

Purpose of the Study:

  • To develop a rapid and efficient protocol for primary hepatocyte isolation.
  • To validate the protocol's effectiveness in supporting in vitro liver metabolism studies.
  • To analyze each step for potential future optimization.

Main Methods:

  • Comparative analysis of existing primary hepatocyte isolation protocols.
  • Development of a streamlined protocol by combining advantages of different methods.
  • Validation of isolated primary hepatocyte quality and function through glucose metabolism experiments.

Main Results:

  • A novel primary hepatocyte isolation protocol was successfully formulated.
  • The protocol isolates healthy primary hepatocytes in approximately 35 minutes.
  • Isolated hepatocytes demonstrated suitability for in vitro glucose metabolism studies.

Conclusions:

  • The developed protocol offers a significant improvement in efficiency for primary hepatocyte isolation.
  • This rapid method supports high-throughput in vitro liver research, including metabolism studies.
  • Detailed analysis of protocol steps provides a foundation for further refinement.