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A novel BRET based genetic coded biosensor for apoptosis detection at deep tissue level in live animal
Yueling Zhang1, Juan Hu2, Meijuan Yu1
1The Key Laboratory of Developmental Genes and Human Disease, Institute of Life Sciences, Southeast University, # 2 Sipailou Road, Nanjing, 210096, Jiangsu Province, China.
Apoptosis : an International Journal on Programmed Cell Death
|November 8, 2021
Summary
Researchers developed a novel biosensor for detecting apoptosis, or programmed cell death. This tool uses ANNEXIN V and Bioluminescence Resonance Energy Transfer (BRET) for both in vitro and in vivo imaging of early apoptotic events.
Area of Science:
- Biochemistry
- Cell Biology
- Biotechnology
Background:
- ANNEXIN V binds phosphatidylserine (PS) exposed on cell surfaces during early apoptosis.
- Current ANNEXIN V detection methods are primarily in vitro.
- There is a need for in vivo tools to monitor apoptosis in disease contexts.
Purpose of the Study:
- To develop a novel genetic coded biosensor for apoptosis detection.
- To enable both in vitro and in vivo imaging of apoptotic events.
- To utilize Bioluminescence Resonance Energy Transfer (BRET) for enhanced detection.
Main Methods:
- Fusion of ANNEXIN V with a BRET-optimized NanoLuc luciferase (teLuc).
- Development of a BRET-based genetic coded biosensor.
- Application of the biosensor for in vitro assays and in vivo animal imaging.
Main Results:
- The new biosensor facilitates both in vitro fluorescence-based and in vivo bioluminescence-based apoptosis detection.
- The robust bioluminescence signal allows for deep tissue imaging in live animals.
- The sensor enables evaluation of disease-induced apoptotic damage and recovery.
Conclusions:
- A novel BRET-based ANNEXIN V biosensor provides a versatile tool for apoptosis detection.
- This technology bridges in vitro and in vivo studies, offering new possibilities for disease monitoring.
- The biosensor's capability for deep tissue imaging in live animals is a significant advancement.
