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Updated: Oct 14, 2025

The bm12 Inducible Model of Systemic Lupus Erythematosus SLE in C57BL/6 Mice

The bm12 Inducible Model of Systemic Lupus Erythematosus SLE in C57BL/6 Mice

Published on: November 1, 2015

Assessing Lupus-Like Disease in Murine Model Systems.

Hui Yin Lee¹, Teja Celhar², Anna-Marie Fairhurst^1,2,3

¹Institute of Molecular and Cellular Biology (IMCB), Agency of Science, Technology and Research, Singapore.

Current Protocols

|November 8, 2021

Summary

This study details methods for evaluating immunological features in Systemic Lupus Erythematosus (SLE) using murine models. These protocols aid in understanding disease mechanisms and developing novel therapeutics for autoimmune conditions.

Keywords:

SLE animal models immune system immunology lupus

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Area of Science:

Immunology
Autoimmune Diseases
Genetics

Background:

Systemic Lupus Erythematosus (SLE) is a complex autoimmune disease influenced by genetic, environmental, and immunological factors.
Murine models are crucial for studying SLE mechanisms and therapeutic development.
Key disease indicators include anti-nuclear autoantibodies (ANAs), splenomegaly, and kidney disease, all linked to immune dysregulation.

Purpose of the Study:

To describe methods for evaluating immunological features in SLE.
To provide a sensitive strategy for understanding SLE pathogenesis.
To explore mechanisms of potential novel therapeutics for SLE.

Main Methods:

Tissue harvesting and processing protocols for murine models.
Immunophenotyping using flow cytometry.
Analysis of serum autoantibodies (ANAs, snRNP, dsDNA) via ELISAs.

Main Results:

Detailed protocols for comprehensive immunological assessment in SLE murine models.
Methods enable sensitive evaluation of immune dysregulation underlying SLE.
Established protocols support research into novel SLE therapeutic strategies.

Conclusions:

Evaluating immunological features offers a sensitive approach to understanding SLE.
Standardized methods are essential for reproducible research in SLE.
These protocols facilitate the investigation of novel therapeutic targets and treatments for SLE.