Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

High-Resolution Mass Spectrometry (HRMS)01:15

High-Resolution Mass Spectrometry (HRMS)

1.8K
The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
1.8K
Gas Chromatography–Mass Spectrometry (GC–MS)01:14

Gas Chromatography–Mass Spectrometry (GC–MS)

5.1K
Gas chromatography–mass spectrometry (GC–MS) is the combination of analytical techniques of gas chromatography and mass spectrometry in a single instrument for analyzing a mixture of compounds. The gas chromatograph separates the compounds in the mixture, and the mass spectrometer analyzes each compound separately to determine the molecular masses and molecular structures.
A gas chromatograph consists of a long, narrow capillary column with a polysiloxane coating on the inner wall....
5.1K
Gas Chromatography: Overview of Detectors01:13

Gas Chromatography: Overview of Detectors

1.0K
Detectors in gas chromatography (GC) help identify and quantify the components of a mixture by translating chemical properties into measurable signals, which are displayed on a chromatogram. Detectors can be categorized into two main types: destructive and non-destructive.
A non-destructive detector allows a sample to be analyzed without altering or consuming it, meaning the sample can be collected after detection for further analysis. Examples include thermal conductivity detectors and...
1.0K
Gas Chromatography: Types of Detectors-II01:19

Gas Chromatography: Types of Detectors-II

647
In gas chromatography, different detectors are employed to meet specific analytical needs. These detectors are often categorized based on their detection mechanisms and the types of compounds they are best suited to analyze. Thermal Conductivity Detectors (TCD), Flame Ionization Detectors (FID), and Electron Capture Detectors (ECD) represent common categories, each with unique operating principles and applications. However, beyond these, several other detectors are designed for more specialized...
647
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

1.1K
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
1.1K
Mass Analyzers: Overview01:13

Mass Analyzers: Overview

991
The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy electron beam to generate a radical cation and further fragment into neutral molecules, radicals, and cations. A series of negatively charged accelerator plates accelerate the cations into the mass analyzer. The mass analyzer separates ions according to their mass-to-charge (m/z) ratios and then directs them to the detector. The common types of mass...
991

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genetic profiling enhances cystic fibrosis prenatal diagnosis.

Scientific reports·2026
Same author

Hexahydroquinoline Featuring Amide Functionality: A Promising Scaffold With Calcium Channel Blocking Activity.

Drug development research·2026
Same author

A Distinct Anion Separation Approach via Isocratic Liquid Chromatography Coupled with Vacuum Ultraviolet Detection.

Analytical chemistry·2026
Same author

Mirror-Image β-l-Cyclodextrin as a Chiral Pseudophase in Capillary Electrophoresis.

Electrophoresis·2025
Same author

Synthesis and Isomerization of Tetrahydro-4<i>H</i>-chromene Derivatives.

The Journal of organic chemistry·2025
Same author

Selective Chemometric Elimination of Co-Eluting Components in Chiral and Achiral Liquid Chromatographic Analyses.

Analytical chemistry·2025

Related Experiment Video

Updated: Oct 14, 2025

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures
09:38

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Published on: January 7, 2019

8.8K

Enhancing Sensitivity for High-Selectivity Gas Chromatography-Molecular Rotational Resonance Spectroscopy.

M Farooq Wahab1, Saba Aslani1, Alexander V Mikhonin2

  • 1Department of Chemistry & Biochemistry, University of Texas at Arlington, Arlington, Texas 76019, United States.

Analytical Chemistry
|November 8, 2021
PubMed
Summary

A new gas chromatograph-molecular rotational resonance (GC-MRR) spectrometer offers higher sensitivity for molecule detection. This advanced system provides detailed structural information, surpassing other analytical techniques for identifying compounds.

More Related Videos

Quantitative Detection of Trace Explosive Vapors by Programmed Temperature Desorption Gas Chromatography-Electron Capture Detector
07:57

Quantitative Detection of Trace Explosive Vapors by Programmed Temperature Desorption Gas Chromatography-Electron Capture Detector

Published on: July 25, 2014

20.2K
Characterization of Synthetic Polymers via Matrix Assisted Laser Desorption Ionization Time of Flight MALDI-TOF Mass Spectrometry
06:56

Characterization of Synthetic Polymers via Matrix Assisted Laser Desorption Ionization Time of Flight MALDI-TOF Mass Spectrometry

Published on: June 10, 2018

25.7K

Related Experiment Videos

Last Updated: Oct 14, 2025

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures
09:38

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Published on: January 7, 2019

8.8K
Quantitative Detection of Trace Explosive Vapors by Programmed Temperature Desorption Gas Chromatography-Electron Capture Detector
07:57

Quantitative Detection of Trace Explosive Vapors by Programmed Temperature Desorption Gas Chromatography-Electron Capture Detector

Published on: July 25, 2014

20.2K
Characterization of Synthetic Polymers via Matrix Assisted Laser Desorption Ionization Time of Flight MALDI-TOF Mass Spectrometry
06:56

Characterization of Synthetic Polymers via Matrix Assisted Laser Desorption Ionization Time of Flight MALDI-TOF Mass Spectrometry

Published on: June 10, 2018

25.7K

Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Physical Chemistry

Background:

  • Molecular Rotational Resonance (MRR) spectroscopy provides rich structural information with high specificity.
  • Previous GC-MRR systems demonstrated the technique's potential but lacked sensitivity.
  • Advanced analytical methods are crucial for detailed molecular structure elucidation.

Purpose of the Study:

  • To describe a next-generation gas chromatograph-molecular rotational resonance (GC-MRR) spectrometer with enhanced sensitivity and instrumental improvements.
  • To showcase the system's capability in analyzing a diverse range of molecules.
  • To explore novel aspects of GC-MRR, including carrier gas effects and total molecule monitoring.

Main Methods:

  • Integration of a Fabry-Pérot cavity and a supersonic jet into the GC-MRR system.
  • Utilizing a supersonic jet to cool analytes to approximately 2 K, reducing spectral complexity.
  • Testing the system's performance with various molecules possessing permanent dipole moments.

Main Results:

  • Achieved significant sensitivity improvements for molecules up to 244 Da in the microwave region.
  • Demonstrated GC-MRR detection limits comparable to a GC thermal conductivity detector.
  • Reported the lowest mass detection limit for any substance by MRR to date.
  • Observed unexpected effects of GC carrier gas on analysis sensitivity.
  • Illustrated the concept of total molecule monitoring in GC-MRR.

Conclusions:

  • The next-generation GC-MRR system offers superior sensitivity and structural specificity for molecular analysis.
  • The incorporation of a supersonic jet is key to enhancing signal strength and enabling lower detection limits.
  • Further research into carrier gas effects and total molecule monitoring can expand GC-MRR applications.