Related Experiment Video
Updated: Oct 14, 2025
Signal Acquisition, Score Interpretation, and Economics of a Non-Invasive Point-of-Care Test for Coronary Artery Disease
Published on: August 9, 2024
Point of Care Test Technology Suitable for Early Detection and Monitoring of Ischemic Stroke
Young Mi Lee1, Mi Jung Bae1, Ye Seul Choi1
1Department of Physiology, School of Medicine, Kyungpook National University, Daegu 41405,Korea.
Insights
A novel filter system detects soluble P-selectin, a stroke biomarker, using quantum dots for early ischemic stroke diagnosis. This method simplifies analysis and enhances sensitivity for point-of-care testing.
Area of Science:
- Biomarker detection
- Nanotechnology applications
- Point-of-care diagnostics
Background:
- Stroke is a major cause of adult death and disability globally.
- Early detection and monitoring of high-risk patients are crucial for stroke management.
- Soluble P-selectin is a key biomarker for platelet aggregation, elevated in stroke and other cardiovascular diseases.
Purpose of the Study:
- To develop a simple, sensitive method for detecting elevated soluble P-selectin levels in stroke patients.
- To enable early diagnosis, hospital monitoring, and risk assessment for ischemic stroke.
- To create a point-of-care test (POCT) that complements existing diagnostic capabilities.
Main Methods:
- A three-layered filter system was developed to separate plasma proteins from whole blood.
- Quantum dots conjugated with antibodies were used to detect soluble P-selectin.
- Fluorescence spectrophotometry was employed for quantitative measurement of the biomarker.
Main Results:
- The developed system achieved a lower limit of detection for soluble P-selectin at 10 pg/ul.
- Saturation signal intensity was observed at 5 ng/ul, indicating the upper detection limit.
- The method demonstrated high sensitivity and simplified the analytical process.
Conclusions:
- A novel three-layer filter membrane system combined with quantum dot-labeled antibodies enables sensitive biomarker detection.
- This approach simplifies blood analysis and holds potential for point-of-care testing in clinical settings.
- The proposed system aims to improve stroke diagnosis and patient monitoring through accessible technology.
Background:
Stroke is one of the leading causes of death and disability in adulthood worldwide. A simple and convenient diagnostic method is needed for monitoring high-risk patients for stroke. Few POCTs are available for stroke diagnosis. Soluble blood P-selectin is known as a biomarker for platelet aggregation. Increased expression of P-selectin is observed in coronary artery disease, acute myocardial infarction, stroke and peripheral arterial disease.
Objective:
A simple method that can measure the increased expression of P-selectin in stroke patients is intended to be used for diagnosis or early detection and hospital monitoring of ischemic stroke.
Methods:
Plasma proteins in blood were separated using a three-layered filter system. Quantum dot and antibody were conjugated to detect biomarkers present in plasma and then measured with a fluorescence spectrophotometer.
Results:
The detection limit of soluble P-selectin confirmed by immunoassay was 1 ng/ul. In order to increase the sensitivity and simplify the reaction, the detection limit was measured to evaluate the sensitivity of the quantum dot labeled anti P-selectin antibody. As a result, P-selectin of 5 ng/ul or more showed saturation signal intensity, indicating the upper limit of detection, and 10 pg/ul was the lower limit of detection.
Conclusion:
In this study, we proposed a three-layer filter membrane system that can separate biomarker- rich fractions from whole blood, simplifying the analysis process and improving sensitivity by using quantum dot-labeled antibodies to detect biomarkers. We hope that our system complements the advantages of POCT and can be applied to real clinical applications.
More Related Videos
04:01Point of Care Transcranial Color-Coded Duplex Ultrasound of the Middle Cerebral Artery
Published on: August 9, 2024
06:45Author Spotlight: Integrated Photoacoustic, Ultrasound, and Angiographic Tomography (PAUSAT) for NonInvasive Whole-Brain Imaging of Ischemic Stroke
Published on: June 2, 2023
Related Concept Videos
01:30Pulse rhythm
Conversely, an irregular pulse pattern is termed dysrhythmia, stemming from disruptions in cardiac...
01:30Acute Coronary Syndrome III: Diagnostic Studies