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Updated: Oct 14, 2025

Author Spotlight: The Production of Recombinant Proteins
Published on: June 30, 2023
Starting a new recombinant protein production project in Escherichia coli
Dianela Aguilar Lucero1, Alejo Cantoia1, Eduardo A Ceccarelli1
1Instituto de Biología Molecular y Celular de Rosario (IBR), CONICET, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina.
Abstract:
One of the goals in recombinant protein production in Escherichia coli is to maximize productivity. High volumetric and specific yields can be reached after careful selection of expression strains and optimization of cultivation parameters. In this chapter, we review the many tools available to make the most out of this versatile microbial cell factory. Useful guidelines and options for troubleshooting production are presented.

