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Published on: March 24, 2022
Optical clearing of tissues: Issues of antimicrobial phototherapy and drug delivery
Valery V Tuchin1, Elina A Genina2, Elena S Tuchina3
1Science Medical Center, Saratov State University, Saratov 410012, Russian Federation; Institute of Physics, Saratov State University, Saratov 410012, Russian Federation; Laboratory of Laser Molecular Imaging and Machine Learning, National Research Tomsk State University, Tomsk 634050, Russian Federation; Laboratory of Laser Diagnostics of Technical and Living Systems, Institute of Precision Mechanics and Control of the Russian Academy of Sciences, Saratov 410028, Russian Federation; A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow 119071, Russian Federation.
Abstract:
This review presents principles and novelties in the field of tissue optical clearing (TOC) technology, as well as application for optical monitoring of drug delivery and effective antimicrobial phototherapy. TOC is based on altering the optical properties of tissue through the introduction of immersion optical cleaning agents (OCA), which impregnate the tissue of interest. We also analyze various methods and kinetics of delivery of photodynamic agents, nanoantibiotics and their mixtures with OCAs into the tissue depth in the context of antimicrobial and antifungal phototherapy. In vitro and in vivo studies of antimicrobial phototherapies, such as photodynamic, photothermal plasmonic and photocatalytic, are summarized, and the prospects of a new TOC technology for effective killing of pathogens are discussed.
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