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Related Experiment Video

Updated: Oct 14, 2025

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Analixity: An open source, low-cost analysis system for the elevated plus maze test, based on computer vision

Patricia González-Gaspar1, Monserrat Macías-Carballo2, Teresa Cadena-Mejía3

  • 1Universidad Veracruzana, Facultad de Matemáticas, Mexico; Universidad Veracruzana, Instituto de Investigaciones en Inteligencia Artificial, Mexico.

Behavioural Processes
|November 9, 2021
PubMed
Summary

Analixity is a new open-source software for automated rodent behavioral analysis in the elevated plus maze (EPM) test. It reduces analysis time and variability, providing results comparable to manual analysis and commercial software.

Keywords:
BehaviorElevated plus mazeImage processingOpen sourceSoftwareVideo analysis

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Area of Science:

  • Neuroscience
  • Behavioral Science
  • Software Engineering

Background:

  • Manual analysis of rodent behavioral tests introduces variability.
  • Automated analysis is needed to improve reliability and efficiency.

Purpose of the Study:

  • Introduce Analixity, an open-source software for automated elevated plus maze (EPM) analysis.
  • Validate Analixity's accuracy against manual analysis and commercial software.

Main Methods:

  • Developed Analixity, a multiplatform, open-source video processing software.
  • Quantified behavioral parameters including time in zones and entries.
  • Compared Analixity results with manual scoring and ANY-maze software.

Main Results:

  • Analixity automated the quantification of EPM behavioral parameters.
  • No statistically significant differences were found between Analixity and manual analysis.
  • Analixity showed comparable results to the commercial software ANY-maze.

Conclusions:

  • Analixity is a reliable and effective open-source alternative for EPM behavioral analysis.
  • The software reduces analysis time and variability.
  • Analixity offers a cost-effective solution for researchers.