Drought stress memory in rice guard cells: Proteome changes and genomic stability of DNA

Drought is one of the major threats for crop plants among them rice, worldwide. The effects of drought vary depending on the plant growth phase and the occurrence of a previous stress, which can leave a memory of the stress. Stomata guard cells perform many essential functions and are highly responsive to hormonal and environmental stimuli. Therefore, information on how guard cells respond to drought might be useful for selecting drought tolerant plants. In this work, physiological analysis, comparative proteomics, gene expression and 5 - methylcytosine (%) analysis were used to elucidate the effects of drought in single stress event at vegetative or reproductive stage or recurrent at both stages in guard cells from rice plants. Photosynthesis and stomatal conductance decreased when drought was applied at reproductive stage in single and recurrent event. Twelve drought-responsive proteins were identified, belonging to photosynthesis pathway, response to oxidative stress, stress signalling and others. The expression of their encoding genes showed a positive relation with the protein abundance. Drought stress increased the total DNA methylation when applied at vegetative stage in single (35%) and recurrent event (18%) and decreased it in plants stressed at reproductive stage (9.8%), with respect to the levels measured in well-watered ones (13.84%). In conclusion, a first drought event seems to induce adaptation to water-deficit conditions through decreasing energy dissipation, increasing ATP energy provision, reducing oxidative damage in GC. Furthermore, the stress memory is associated with epigenetic markers.