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Optimization of five qPCR protocols toward the detection and the quantification of antimicrobial resistance genes in
Roberta Tolosi1, Lisa Carraro1, Andrea Laconi1
1Department of Comparative Biomedicine and Food Science, University of Padua, Viale dell'Università 16, Legnaro, Padua 35020, Italy.
Abstract:
Here, we describe the optimization and validation of five quantitative PCR (qPCR) assays by employing the SYBRGreen chemistry paired with melting curve analysis to detect and quantify clinically relevant antimicrobial resistance genes (ARGs) (i.e. ermB, bla and qnrS) from environmental samples (i.e. soil and manure). These five protocols accurately detected and quantified the aforementioned ARGs in complex environmental matrices and represent useful tools for both diagnostic and monitoring activities of resistant bacteria and ARGs into the environment.
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