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Updated: Oct 14, 2025

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Upregulation of CD271 transcriptome in breast cancer promotes cell survival via NFκB pathway

Nabiha Bashir1, Mehreen Ishfaq1, Kehkashan Mazhar2

  • 1Department of Biosciences, COMSATS University Islamabad (CUI), Islamabad, Pakistan.

Molecular Biology Reports
|November 10, 2021
PubMed
Abstract

Insights

This study reveals CD271 protein is upregulated in breast cancer, driving cancer progression. Inhibiting CD271 reduces NF-κB pathway activity, impacting cell proliferation and migration, suggesting CD271 as a therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Targeting cell surface receptors is key in cancer treatment.
  • Novel membrane proteins are needed to understand breast cancer metastasis.
  • CD271, a TNF receptor family member, influences cancer cell proliferation.

Purpose of the Study:

  • Investigate the role of CD271 in breast cancer progression.
  • Determine the relationship between CD271 and cancer cell migration/metastasis.

Main Methods:

  • Analyzed CD271 mRNA expression using RT-qPCR in tumor tissue, MCF7 cells, and MCF7-CSCs.
  • Measured CD271 protein levels via Western blotting in MCF-7 cells.
  • Examined CD271 promoter region for NF-κB1 binding sites and quadruplex sequences.

Main Results:

  • CD271 mRNA and protein were upregulated in breast cancer samples and cell lines.
  • CD271 expression correlated with NF-κB pathway activation.
  • Targeting CD271's quadruplex sequence inhibited NF-κB, Nanog, and Sox2 expression.

Conclusions:

  • CD271 and NF-κB signaling are interdependent in breast cancer.
  • CD271 inhibition reduces NF-κB, affecting cell proliferation and migration.
  • CD271 represents a promising therapeutic target for breast cancer treatment.
Keywords:
Breast cancerBreast cancer stem cellsCD271G-quadraplexMCF7-CSCsNF-κB1

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