Ageing affects plasma steroid concentrations and testicular volume, echotexture and haemodynamics in rams

The objective was to determine the effect of ageing on plasma steroid concentrations and testicular volume, echotexture and haemodynamics in Ossimi rams. Twenty-four rams were allocated, by age, into three groups: young (1 year; 32.00 ± 0.78 kg, n = 6); mature (2.5 years; 48.00 ± 1.02 kg, n = 9) and aged (7.5 years; 65.00 ± 1.10 kg, n = 9). Aged rams had the highest (p ˂ 0.05) plasma testosterone concentrations 3.90 ± 0.75 ng/ml (three-fold the other groups), whereas in young rams, plasma estradiol-17β concentrations (5.85 ± 0.34 pg/ml) were approximately one-quarter (p ˂ 0.05) of other groups. Regarding pulsed-wave Doppler indices values of the (resistive index RI; and pulsatility index PI) were lower (p ˂ 0.05) in the mature (0.32 ± 0.04 and 0.36 ± 0.03, respectively) and aged (0.51 ± 0.03 and 0.77 ± 0.06) rams compared to young rams (0.62 ± 0.03 and 0.98 ± 0.07). In the young group, testicular pixel intensity was highest (p ˂ 0.05) but values of coloured area/pixels were lowest (p ˂ 0.05) compared to the other groups. In conclusion, these parameters indicate that aged rams (7.5 years) are likely to be suitable for breeding ahead of the younger aged groups (1 and 2.5 years) examined, but a final decision would need to be tempered by consideration of other parameters in the breeding soundness examination (BSE), such as physical soundness and semen characteristics. Of the parameters studied, testicular pixel intensity could be most readily incorporated in a BSE for deciding a ram's breeding efficiency.