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Published on: July 3, 2013
Morphofunctional study on the effects of passive smoking in kidneys of rats
Carlos Alberto de Moraes1, Mercia Breda-Stella1, Cesar Alexandre Fabrega Carvalho1
1Faculdade de Medicina de Jundiaí, Jundiaí, SP, Brazil.
Objective:
To analyze whether passive inhalation of cigarette smoke causes morphological, structural, and functional changes in kidneys of rats.
Methods:
Wistar rats, aged eight weeks, weighing on average 260g, were divided into Control Group and Smoking Group. Each group was subdivided into four groups of ten animals for morphofunctional analysis, in a period of seven and 28 days. The Smoking Group was exposed to smoke of 40 cigarettes per day, at certain times and in automated equipment for cigarette burning, called smoking machine (SM-MC-01). After the exposure period, urine and blood samples were collected for the functional analyses, and the kidneys were dissected and submitted to histological procedures for morphoquantitative analyses.
Results:
After exposure of animals of the Smoking Group, the following were observed: lower weight gain; lower water and feed intake; decreased renal weight, diameter, and volume; reduction in cortical thickness and glomerular volume density; decrease in glomerular and capsular diameter; increase in mesangial density; decreased urine volume; increased levels of glucose, serum creatinine and microalbuminuria; decreased urinary creatinine levels and creatinine clearance rate.
Conclusion:
Passive smoking negatively influences renal morphology and glomerular filtration rate, with effects similar to those described in the literature regarding active smoking.
