Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

T Cell Types and Functions01:24

T Cell Types and Functions

1.5K
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
1.5K
Differentiation of Common Myeloid Progenitor Cells01:15

Differentiation of Common Myeloid Progenitor Cells

3.4K
Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
3.4K
B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

9.8K
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
9.8K
Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

3.4K
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
3.4K
Role of Hematopoietic Growth Factors01:28

Role of Hematopoietic Growth Factors

1.9K
Hematopoietic growth factors are molecules that regulate the differentiation rate of hematopoietic stem cells (HSCs). Erythropoietin (EPO), primarily produced by the kidneys, plays a crucial role in erythrocyte production. When oxygen levels in the blood are low, EPO is released into the bloodstream, reaching the bone marrow, where it stimulates HSCs to differentiate and mature into erythrocytes, which are vital for oxygen transport.
Thrombopoietin (TPO), mainly released by the liver,...
1.9K
Lineage Commitment01:21

Lineage Commitment

3.5K
Commitment is the  process whereby stem cells:
3.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Combined large and small airway obstruction is associated with increased exacerbation frequency and poorer symptom control in persistent asthma.

The journal of allergy and clinical immunology. In practice·2026
Same author

Multiple sclerosis patients under treatment with interferon β1-a or ocrelizumab exhibit different T and B cell responses to SARS-CoV-2 vaccine.

Frontiers in immunology·2026
Same author

Allergen Immunotherapy and Possible Clinical Remission: Toward a Disease-Modifying Paradigm in Allergic Disorders.

Biomedicines·2026
Same author

Sialylation Modulates Basophil Responsiveness to Galectin-3-dependent Activation.

Journal of leukocyte biology·2026
Same author

Small Airway Dysfunction May Mediate the Association Between Body Mass Index and Severe Asthma Exacerbations.

The European respiratory journal·2026
Same author

Vascular remodeling in asthma: from mechanisms to precision medicine.

Current opinion in allergy and clinical immunology·2026

Related Experiment Video

Updated: Oct 13, 2025

Basophil Activation Test for Allergy Diagnosis
07:22

Basophil Activation Test for Allergy Diagnosis

Published on: May 31, 2021

8.6K

IL-3 in the development and function of basophils.

Gilda Varricchi1, Remo Poto2, Gianni Marone1

  • 1Department of Translational Medical Sciences, University of Naples Federico II, 80131, Naples, Italy; Center for Basic and Clinical Immunology Research (CISI), University of Naples Federico II, 80131, Naples, Italy; World Allergy Organization (WAO) Center of Excellence, 80131, Naples, Italy; Institute of Experimental Endocrinology and Oncology (IEOS), National Research Council, 80131, Naples, Italy.

Seminars in Immunology
|November 10, 2021
PubMed
Summary

Interleukin-3 (IL-3) is a key cytokine that activates immune cells like basophils and mast cells, contributing to allergic and inflammatory conditions. Targeting the IL-3 receptor alpha (IL-3Rα/CD123) shows promise for treating hematologic malignancies.

Keywords:
AsthmaBasophilCancerGM-CSFIL-3IL-33

More Related Videos

A Strategy for the Study of IL-9-Producing Lymphoid Cells in the Nippostrongylus brasiliensis Infection Model
08:38

A Strategy for the Study of IL-9-Producing Lymphoid Cells in the Nippostrongylus brasiliensis Infection Model

Published on: March 3, 2023

1.5K
Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity
10:22

Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity

Published on: September 16, 2011

33.8K

Related Experiment Videos

Last Updated: Oct 13, 2025

Basophil Activation Test for Allergy Diagnosis
07:22

Basophil Activation Test for Allergy Diagnosis

Published on: May 31, 2021

8.6K
A Strategy for the Study of IL-9-Producing Lymphoid Cells in the Nippostrongylus brasiliensis Infection Model
08:38

A Strategy for the Study of IL-9-Producing Lymphoid Cells in the Nippostrongylus brasiliensis Infection Model

Published on: March 3, 2023

1.5K
Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity
10:22

Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity

Published on: September 16, 2011

33.8K

Area of Science:

  • Immunology
  • Cell Biology
  • Hematology

Background:

  • The beta common (βc) cytokine family, including IL-3, GM-CSF, and IL-5, utilizes the βc receptor subunit for signaling.
  • Interleukin-3 (IL-3) signals through IL-3 receptor alpha (IL-3Rα/CD123) and the βc subunit.
  • IL-3 is produced by various immune and non-immune cells and acts as a critical growth and activating factor for basophils and mast cells.

Purpose of the Study:

  • To elucidate the multifaceted roles of IL-3 in immune responses and disease pathogenesis.
  • To highlight the involvement of IL-3-activated cells in allergic disorders, inflammation, infections, and cancer.
  • To review the therapeutic potential of targeting IL-3Rα/CD123 in hematologic malignancies.

Main Methods:

  • Literature review and synthesis of existing research on IL-3 and its receptor.
  • Analysis of IL-3's cellular sources and target cells.
  • Examination of IL-3's functional effects on immune cells and its pathological implications.

Main Results:

  • IL-3 is crucial for basophil and mast cell activation, driving allergic reactions and inflammation.
  • IL-3-activated basophils release pro-inflammatory cytokines (e.g., IL-4, IL-13, CXCL8) and potentiate responses to other stimuli.
  • IL-3 plays a pathogenic role in hematologic cancers and may contribute to autoimmune and cardiac diseases.

Conclusions:

  • IL-3 is a significant mediator in allergic disorders, chronic inflammation, and hematologic malignancies.
  • Targeting IL-3Rα/CD123 represents a promising therapeutic strategy for hematologic cancers.
  • Further research into IL-3 signaling pathways could uncover new therapeutic avenues.