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Updated: Oct 13, 2025
High-Throughput DNA Plasmid Multiplexing and Transfection Using Acoustic Nanodispensing Technology
Published on: August 8, 2019
Fully Integrated Self-Powered Electrical Stimulation Cell Culture Dish for Noncontact High-Efficiency Plasmid
Miaomiao Yuan1, Xiaoying Li2, Jingfeng Liu2,3
1The Eighth Affiliated Hospital, Sun Yat-sen University, Shenzhen 518033, China.
Abstract:
Plasmid DNA transfection of mammalian cells is widely used in biomedical research and genetic drug delivery, but low transfection efficiency, especially in the context of the primary cells, limits its application. To improve the efficiency of plasmid transfection, a fully integrated self-powered electrical stimulation cell culture dish (SESD) has been developed to provide self-powered electrical stimulation (ES) of adherent cells, significantly improving the efficiency of plasmid transfection into mammalian cells and cell survival by the standard lipofectamine transfection method. Mechanistically, ES can safely increase the intracellular calcium concentration by opening calcium-ion channels, leading to a higher efficiency of plasmid transfection. Therefore, SESD has the potential to become an effective platform for high-efficiency plasmid DNA transfection in biomedical research and drug delivery.
