Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

62.0K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
62.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dissipative soliton generation and real-time dynamics in microresonator-filtered fiber lasers.

Light, science & applications·2022
Same author

Gastric cancer-derived exosomal miR-519a-3p promotes liver metastasis by inducing intrahepatic M2-like macrophage-mediated angiogenesis.

Journal of experimental & clinical cancer research : CR·2022
Same author

Effects of different modes of exercise on skeletal muscle mass and function and IGF-1 signaling during early aging in mice.

The Journal of experimental biology·2022
Same author

Optimal Control of False Information Clarification System under Major Emergencies Based on Differential Game Theory.

Computational intelligence and neuroscience·2022
Same author

Ionothermal Synthesis of Fully Conjugated Covalent Organic Frameworks for High-Capacity and Ultrastable Potassium-Ion Batteries.

Advanced materials (Deerfield Beach, Fla.)·2022
Same author

Circ-OMAC drives metastasis in oral squamous cell carcinoma.

Oral diseases·2022

Related Experiment Video

Updated: Oct 13, 2025

Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification
07:54

Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification

Published on: March 31, 2021

4.9K

Duplex Reverse Transcription Multienzyme Isothermal Rapid Amplification Assays for Detecting SARS-CoV-2.

Meiyun Shang, Jin Li, Xiange Sun

    Clinical Laboratory
    |November 10, 2021
    PubMed
    Summary

    A new real-time duplex reverse transcription multienzyme isothermal rapid amplification (RT-MIRA) assay can detect SARS-CoV-2, the virus causing COVID-19, in under 20 minutes. This rapid COVID-19 test is faster and simpler than RT-PCR methods.

    More Related Videos

    Author Spotlight: Advancements in Multiplex Detection of Respiratory Viruses
    03:53

    Author Spotlight: Advancements in Multiplex Detection of Respiratory Viruses

    Published on: November 10, 2023

    1.5K
    Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP
    05:34

    Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP

    Published on: September 8, 2023

    940

    Related Experiment Videos

    Last Updated: Oct 13, 2025

    Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification
    07:54

    Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification

    Published on: March 31, 2021

    4.9K
    Author Spotlight: Advancements in Multiplex Detection of Respiratory Viruses
    03:53

    Author Spotlight: Advancements in Multiplex Detection of Respiratory Viruses

    Published on: November 10, 2023

    1.5K
    Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP
    05:34

    Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP

    Published on: September 8, 2023

    940

    Area of Science:

    • Virology
    • Molecular Biology
    • Biotechnology

    Background:

    • Coronavirus disease 2019 (COVID-19), caused by SARS-CoV-2, presents a significant global public health challenge.
    • Rapid and accessible diagnostic methods are crucial for effective population screening and sample testing.

    Purpose of the Study:

    • To develop and describe a novel real-time duplex RT-MIRA assay for detecting SARS-CoV-2.
    • To establish a rapid and sensitive method for identifying the nucleocapsid protein gene (N gene) of SARS-CoV-2.

    Main Methods:

    • The study employed real-time duplex reverse transcription multienzyme isothermal rapid amplification (RT-MIRA) assays.
    • Detection targeted the SARS-CoV-2 nucleocapsid protein gene (N gene) and the human ACTB gene as an internal control.

    Main Results:

    • The RT-MIRA assay demonstrated high specificity with no observed cross-reactions to other pathogens.
    • A sensitive detection limit of 100 copies/reaction was achieved.
    • The amplification process was completed in under 20 minutes at 42°C, validated with simulated clinical samples.

    Conclusions:

    • RT-MIRA assays offer a faster and simpler alternative to traditional reverse transcription real-time polymerase chain reaction (RT-PCR) for SARS-CoV-2 detection.
    • The developed RT-MIRA assay shows promise for further optimization and clinical evaluation in confirmed COVID-19 cases.