Related Experiment Video
Updated: Oct 13, 2025
Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
Published on: March 30, 2019
Sinomenine Inhibits the Progression of Bladder Cancer Cells by Downregulating LncRNA-HEIH Expression
Haili Xu1, Jiayan Dong2, Junhui Hou3
1Department of Oncology, Jinan Municipal Hospital of Traditional Chinese Medicine, Jinan 250012, China.
Background:
Sinomenine has been reported to effectively repress the progression of lung cancer and breast cancer. However, the effects of sinomenine in bladder cancer are not well understood. The purpose of this study was to evaluate the effects of sinomenine in bladder cancer.
Methods:
The mRNA expression of HEIH in bladder cancer cells was measured by RT-qPCR. T24 and SW780 cells were treated with sinomenine for 24 hours. Cell viability was detected by the MTT assay. Cell migration and invasion were detected by the transwell assay. Western blotting assay was performed to assess the protein expression of Bcl-2, Bax, and caspase-3.
Results:
Sinomenine significantly suppressed cell viability in T24 and SW780 cells. Moreover, cell migration and invasion were significantly inhibited by sinomenine. Sinomenine accelerated the expression of Bax and caspase-3 but decreased the expression of Bcl-2. HEIH was upregulated in bladder cancer cells compared with normal bladder epithelial cells. Besides this, we noticed that HEIH knockdown blocked cell proliferation, migration, and invasion but facilitated cell apoptosis in bladder cancer cells. Additionally, HEIH reversed the suppression of the progression induced by sinomenine.
Conclusion:
Sinomenine was observed to suppress cell progression of bladder cancer cells by inhibiting HEIH expression. Our findings suggested that the use of sinomenine might be an effective treatment for bladder cancer.
Insights
Sinomenine effectively suppresses bladder cancer progression by inhibiting HEIH expression, leading to reduced cell viability, migration, and invasion. This suggests sinomenine as a potential therapeutic agent for bladder cancer treatment.
Area of Science:
- Oncology
- Molecular Biology
- Pharmacology
Background:
- Sinomenine demonstrates anti-cancer properties in lung and breast cancers.
- The therapeutic potential of sinomenine in bladder cancer remains largely unexplored.
Purpose of the Study:
- To investigate the effects of sinomenine on bladder cancer progression.
- To elucidate the underlying molecular mechanisms of sinomenine's action in bladder cancer cells.
Main Methods:
- Quantitative real-time PCR (RT-qPCR) for HEIH mRNA expression.
- Cell viability assays (MTT), migration and invasion assays (Transwell).
- Western blotting for apoptosis-related proteins (Bcl-2, Bax, caspase-3).
Main Results:
- Sinomenine significantly inhibited bladder cancer cell viability, migration, and invasion.
- Sinomenine modulated apoptosis markers, increasing Bax and caspase-3 while decreasing Bcl-2.
- HEIH was upregulated in bladder cancer and its knockdown mimicked sinomenine's effects, with HEIH reversing sinomenine's suppression.
Conclusions:
- Sinomenine suppresses bladder cancer progression, partly through the inhibition of HEIH expression.
- Sinomenine exhibits potential as an effective therapeutic strategy for bladder cancer.
Related Concept Videos
02:39lncRNA - Long Non-coding RNAs
02:57Targeted Cancer Therapies
There are several types of targeted therapies against...
01:22MicroRNAs