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Updated: Oct 13, 2025

In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells
Published on: March 16, 2017
Optimized protocol for naive human pluripotent stem cell-derived trophoblast induction
Shingo Io1,2,3, Yoshiki Iemura1,4,5, Yasuhiro Takashima1
1Department of Life Science Frontiers, CiRA, Kyoto University, Kyoto 606-8507, Japan.
Abstract:
Human trophoblasts arise from the morula as trophectoderm, which differentiates into cytotrophoblast, syncytiotrophoblast, and extravillous trophoblast after implantation. Here, we present a robust step-by-step protocol to induce trophectoderm (TE) from naive human pluripotent stem cells (PSCs) corresponding to pre-implantation epiblast. Our culture system (TE induction and ACE condition) mimics the entire trophoblast development including the molecular events. For complete details on the use and execution of this protocol, please refer to Io et al. (2021).

