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Correlating Corona Composition and Cell Uptake to Identify Proteins Affecting Nanoparticle Entry into Endothelial
Aldy Aliyandi1, Catharina Reker-Smit1, Reinier Bron2
1Department of Nanomedicine & Drug Targeting, Groningen Research Institute of Pharmacy, University of Groningen, Antonius Deusinglaan 1, 9713AV Groningen, The Netherlands.
ACS Biomaterials Science & Engineering
|November 11, 2021
Summary
Understanding the nanoparticle protein corona is key for drug delivery. This study identified specific proteins that can either increase or decrease nanoparticle uptake by cells, offering new ways to control nanocarrier performance.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Cell Biology
Background:
- The biomolecule corona on nanoparticles influences their interaction with cells, impacting drug delivery efficacy.
- Specific corona proteins can mediate nanoparticle interactions with cellular receptors, affecting cellular uptake.
- Controlling nanoparticle uptake is crucial for optimizing nanocarrier performance in therapeutic applications.
Purpose of the Study:
- To identify specific proteins within the nanoparticle corona that modulate cellular uptake.
- To correlate nanoparticle corona composition with uptake efficiency in different endothelial cell types.
- To validate the role of identified proteins in nanoparticle-cell interactions for drug delivery.
Main Methods:
- Correlation analysis between silica nanoparticle corona composition and cellular uptake across four endothelial cell types.
- Investigation of nanoparticle uptake using different sizes and surface functionalities.
- Validation studies using single protein coronas and competition assays in brain and liver endothelium.
Main Results:
- Proteins correlating with increased or decreased nanoparticle uptake were identified.
- Histidine-rich glycoprotein (HRG) precoating significantly reduced nanoparticle uptake in liver and brain endothelium.
- Transferrin receptor involvement in liver endothelium uptake was suggested, with potential redirection to other receptors.
Conclusions:
- Correlating protein corona composition with nanoparticle uptake allows for the identification of key protein regulators.
- Specific proteins like HRG can be utilized to decrease nanoparticle uptake, while others may enhance it.
- Cellular microenvironment and receptor availability influence nanoparticle uptake efficiency, offering strategies for targeted delivery.
