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Related Experiment Videos

Plasma lactoferrin levels in leukaemias.

K Német, V Solti, A Mód

    Clinical and Laboratory Haematology
    |January 1, 1987
    PubMed
    Summary

    A new radioimmunoassay accurately measures plasma lactoferrin. This method helps assess the total blood granulocyte pool and shows altered lactoferrin levels in various leukemias.

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    Area of Science:

    • Biochemistry
    • Hematology
    • Immunology

    Background:

    • Plasma lactoferrin, a key protein, plays a role in immune response.
    • Accurate measurement of lactoferrin is crucial for understanding granulocyte pool dynamics.
    • Existing methods for lactoferrin determination have limitations in reproducibility and sensitivity.

    Purpose of the Study:

    • To develop and validate a solid-phase, one-step radioimmunoassay for plasma lactoferrin.
    • To establish normal plasma lactoferrin ranges in healthy adults.
    • To investigate plasma lactoferrin levels in patients with acute and chronic leukemias.

    Main Methods:

    • Development of a solid-phase, one-step radioimmunoassay for plasma lactoferrin determination.
    • Use of EDTA as an anticoagulant to minimize cellular lactoferrin leakage.
    • Measurement of plasma lactoferrin in healthy adults and leukemia patients.

    Main Results:

    • The radioimmunoassay has a detection limit of 150 micrograms/l.
    • Normal plasma lactoferrin geometric means were 483 micrograms/l in men and 446 micrograms/l in women.
    • Significantly elevated lactoferrin levels were observed in chronic myeloid leukemia, while levels were undetectable in acute leukemias during relapse.

    Conclusions:

    • The developed radioimmunoassay is reliable for plasma lactoferrin measurement.
    • Plasma lactoferrin levels are altered in various types of leukemia, correlating with disease status.
    • Plasma lactoferrin determination may aid in assessing the total blood granulocyte pool (TBGP).

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