A novel E6-like gene, E6-2, affects fruit ripening in tomato.
Jing Kang1, Jun Gong1, Lincheng Zhang1
1Laboratory of Molecular Biology of Tomato, Bioengineering College, Chongqing University, Chongqing, People's Republic of China.
Plant Science : an International Journal of Experimental Plant Biology
|November 12, 2021
Summary
The novel E6-2 gene, regulated by MADS-RIN, is crucial for tomato fruit ripening. Silencing E6-2 delays ripening and alters key metabolic processes, highlighting its role in fruit development.
Area of Science:
- Plant Biology
- Molecular Genetics
- Fruit Ripening
Background:
- Tomato fruit ripening involves complex hormonal and genetic regulation.
- MADS-box transcription factor (MADS-RIN) is a known regulator, but its precise mechanisms require further elucidation.
Purpose of the Study:
- To characterize the novel tomato E6-like gene, E6-2, and investigate its role in fruit ripening.
- To determine the regulatory relationship between MADS-RIN and E6-2.
Main Methods:
- Gene expression analysis in different ripening stages and mutants (Nr, rin).
- In vivo binding assays to confirm MADS-RIN's transcriptional activation of E6-2.
- RNA interference (RNAi) to silence E6-2 expression.
- Biochemical analysis of fruit quality traits (carotenoids, pectin, cellulose, ethylene) and gene expression profiling.
Main Results:
- E6-2 expression is high during ripening and low in Nr and rin mutants, with MADS-RIN directly activating E6-2.
- E6-2 expression is reduced by 1-MCP treatment, indicating its involvement in ethylene-mediated ripening.
- RNAi-mediated silencing of E6-2 delayed ripening, reduced carotenoids and ethylene, and increased pectin, cellulose, starch, and soluble sugars.
- Expression of genes involved in carotenoid biosynthesis, ripening, ethylene biosynthesis, and cell wall metabolism was inhibited in E6-2-silenced lines.
Conclusions:
- E6-2 is a novel, RIN-targeted gene essential for normal tomato fruit ripening.
- E6-2 regulates multiple ripening processes, including pigment accumulation, ethylene production, and cell wall modification.
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