Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rest-activity patterns across development in two mouse models of autism and epilepsy.

Epilepsia open·2026
Same author

iAstrocytes model cytokine influences on complement expression and neuronal network synchronization.

bioRxiv : the preprint server for biology·2026
Same author

Overexpression of key complement regulators in glioblastoma.

PloS one·2026
Same author

Electrochemical Detection of Neuronal Injury in Cell Culture Samples: A Cost-Effective Biosensor for Neurofilament Light Sensing.

Biosensors·2026
Same author

Time-dependent changes in monocyte subsets and gene expression patterns are associated with long-term recovery in patients with ischemic stroke.

Research square·2026
Same author

Microstructure imaging in patients undergoing evaluation for epilepsy surgery or low-grade glioma: Clinical utility of a novel diffusion MRI method.

Epilepsia open·2026

Related Experiment Video

Updated: Oct 13, 2025

Transplantation of Human Stem Cell-Derived GABAergic Neurons into the Early Postnatal Mouse Hippocampus to Mitigate Neurodevelopmental Disorders
05:00

Transplantation of Human Stem Cell-Derived GABAergic Neurons into the Early Postnatal Mouse Hippocampus to Mitigate Neurodevelopmental Disorders

Published on: November 11, 2022

2.6K

Human stem cell-derived GABAergic neurons functionally integrate into human neuronal networks.

Ana Gonzalez-Ramos1, Eliška Waloschková2, Apostolos Mikroulis2

  • 1Epilepsy Center, Department of Clinical Sciences, Lund University Hospital, 22184, Lund, Sweden. ana.gonzalez_ramos@med.lu.se.

Scientific Reports
|November 12, 2021
PubMed
Summary
This summary is machine-generated.

Human embryonic stem cell-derived GABAergic interneurons (hdINs) were generated and shown to be functional. These light-sensitive cells can modulate neuronal network activity, offering potential for epilepsy treatment.

More Related Videos

Homochronic Transplantation of Interneuron Precursors into Early Postnatal Mouse Brains
10:08

Homochronic Transplantation of Interneuron Precursors into Early Postnatal Mouse Brains

Published on: June 8, 2018

8.0K
The Specification of Telencephalic Glutamatergic Neurons from Human Pluripotent Stem Cells
10:49

The Specification of Telencephalic Glutamatergic Neurons from Human Pluripotent Stem Cells

Published on: April 14, 2013

11.1K

Related Experiment Videos

Last Updated: Oct 13, 2025

Transplantation of Human Stem Cell-Derived GABAergic Neurons into the Early Postnatal Mouse Hippocampus to Mitigate Neurodevelopmental Disorders
05:00

Transplantation of Human Stem Cell-Derived GABAergic Neurons into the Early Postnatal Mouse Hippocampus to Mitigate Neurodevelopmental Disorders

Published on: November 11, 2022

2.6K
Homochronic Transplantation of Interneuron Precursors into Early Postnatal Mouse Brains
10:08

Homochronic Transplantation of Interneuron Precursors into Early Postnatal Mouse Brains

Published on: June 8, 2018

8.0K
The Specification of Telencephalic Glutamatergic Neurons from Human Pluripotent Stem Cells
10:49

The Specification of Telencephalic Glutamatergic Neurons from Human Pluripotent Stem Cells

Published on: April 14, 2013

11.1K

Area of Science:

  • Neuroscience
  • Stem Cell Biology
  • Epilepsy Research

Background:

  • Gamma-aminobutyric acid (GABA)-releasing interneurons are crucial for maintaining brain excitatory-inhibitory balance.
  • Disruption of this balance is implicated in neurological disorders, including epilepsy.
  • Cell-based therapy presents a potential therapeutic strategy for restoring neuronal function.

Purpose of the Study:

  • To generate and characterize light-sensitive human embryonic stem cell (hESC)-derived GABAergic interneurons (hdINs).
  • To assess the functional integration and modulatory capacity of hdINs in human neuronal networks.
  • To provide a proof-of-concept for using light-sensitive interneurons in cell-based therapies for neurological disorders.

Main Methods:

  • Generation of light-sensitive hESC-derived GABAergic interneurons (hdINs) expressing channelrhodopsin-2 (ChR2).
  • Electrophysiological characterization of hdINs at 35 days in vitro (DIV).
  • Co-culture and transplantation of hdINs into human cortical neurons and epilepsy patient-derived brain tissue.

Main Results:

  • hdINs exhibited electrophysiological properties and spontaneous synaptic currents comparable to mature neurons by 35 DIV.
  • Light-activated hdINs induced postsynaptic currents in human neurons in co-culture and after transplantation.
  • Evidence of functional efferent synapse formation between transplanted hdINs and host neurons was observed.

Conclusions:

  • hESC-derived GABAergic interneurons can functionally integrate into human neuronal networks.
  • Transplanted light-sensitive interneurons demonstrate the potential to modulate host neuronal network activity.
  • This study supports the feasibility of using optogenetically modified hESC-derived neurons for precise control of neural excitability.