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p82H identifies sequences at every human centromere.

C Aleixandre, D A Miller, A R Mitchell

    Human Genetics
    |September 1, 1987
    PubMed
    Summary
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    A cloned alphoid DNA sequence, p82H, binds to the centromere of all human chromosomes, indicating conserved centromeric sequences across the genome. This sequence remains associated with centromeres even after relocation, confirming its centromeric localization.

    Area of Science:

    • * Molecular Biology
    • * Genetics
    • * Cytogenetics

    Background:

    • * Centromeric DNA sequences are crucial for chromosome segregation during cell division.
    • * Previous studies identified chromosome-specific centromeric sequences.
    • * The function and conservation of centromeric sequences require further investigation.

    Purpose of the Study:

    • * To characterize a novel cloned alphoid DNA sequence, p82H.
    • * To determine the chromosomal localization and conservation of p82H.
    • * To investigate the association of p82H with centromeric regions.

    Main Methods:

    • * In situ hybridization of the p82H sequence to human chromosomes.
    • * Stringent washing conditions to assess hybridization specificity.

    Related Experiment Videos

  • * Analysis of p82H hybridization in rearranged chromosomes (inv(19), inv(6)).
  • * Examination of p82H hybridization in chromosomes with altered heterochromatin (1qh, 9qh, 16qh) after 5-azacytidine treatment.

    • Main Results:

    • * The p82H sequence hybridizes to the centromere of every human chromosome.
    • * Stringent washing reveals no more than 8% of hybridization signals on any single chromosome, suggesting broad conservation.
    • * p82H remains associated with centromeres in rearranged chromosomes, including inv(19) and inv(6).
    • * Hybridization occurs at the centromere but not in the elongated heterochromatin of chromosomes 1, 9, and 16, with a single exception at the distal 9qh region.

    Conclusions:

    • * The p82H sequence represents a conserved human centromeric DNA element.
    • * p82H is closely associated with the functional centromere, independent of chromosome-specific variations.
    • * This finding provides insights into the conserved nature of centromeric DNA sequences and their role in chromosome structure and function.