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Identification of potential immune-related circRNA-miRNA-mRNA regulatory network in cutaneous squamous cell carcinoma

Yuanfeng Wei1, Xi Yang1, Limin Gao2

  • 1Department of Medical Oncology, Cancer Center, West China Hospital, Sichuan University Chengdu 610041, Sichuan, China.

American Journal of Cancer Research
|November 12, 2021
PubMed

Insights

This study explores circular RNAs (circRNAs) in cutaneous squamous cell carcinoma (cSCC), revealing a network of immune-related genes. These findings offer insights into cSCC development and potential therapeutic targets.

Area of Science:

  • Oncology
  • Immunology
  • Molecular Biology

Background:

  • Circular RNAs (circRNAs) influence tumor progression and immune regulation.
  • The role of circRNAs in cutaneous squamous cell carcinoma (cSCC) immunomodulation is largely unstudied.

Purpose of the Study:

  • To identify differentially expressed circRNAs, miRNAs, and mRNAs in cSCC.
  • To establish a circRNA-mediated competing endogenous RNA (ceRNA) network.
  • To identify and validate hub immune-related genes in cSCC progression.

Main Methods:

  • Differential expression analysis of circRNAs, miRNAs, and mRNAs.
  • Construction of a circRNA-miRNA-mRNA ceRNA network.
  • Identification and validation of hub immune-related genes using immunohistochemistry.
  • Gene Ontology (GO) and KEGG pathway analysis.

Main Results:

  • Identified 54 differentially expressed circRNAs in cSCC.
  • Established a circRNA-miRNA-mRNA ceRNA network.
  • Identified hub immune-related genes predominantly linked to immune responses in cSCC progression.

Conclusions:

  • The identified immune-related circRNA-miRNA-mRNA network aids in understanding cSCC carcinogenesis and progression.
  • Immune-related genes may serve as potential biomarkers and therapeutic targets for cSCC.
Keywords:
Cutaneous squamous cell carcinomacircRNAsimmune-related genes

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