Wide-Field Pixel Super-Resolution Colour Lensfree Microscope for Digital Pathology

Area of Science:

• Biomedical Imaging
• Microscopy
• Optical Physics

Background:

• Whole slide imaging is crucial for pathology but lensfree holography faces challenges in accurate color reconstruction.
• Conventional optical microscopy is limited by field of view and digital focus capabilities.

Purpose of the Study:

• To develop a wide-field pixel super-resolution colour lensfree microscopy method.
• To address the challenge of accurate colour holographic imaging with super-resolution reconstruction.

Main Methods:

• Proposed a method performing wavelength scanning pixel super-resolution and phase retrieval simultaneously on RGB channels.
• Utilized YUV color space conversion to enhance color components and maintain brightness.
• Implemented an average filter to eliminate rainbow artifacts and preserve high-resolution details.

Main Results:

• Achieved high-resolution composite colour images with suppressed holographic reconstruction artifacts.
• Demonstrated effective spatial resolution and colour reconstruction performance.
• Validated the technique on USAF1951, lotus root, and bone marrow smear samples with a field of view >40 mm².

Conclusions:

• The developed lensfree microscopy technique successfully enables high-resolution colour holographic imaging.
• The YUV space processing effectively eliminates artifacts while preserving image details.
• This method offers a promising alternative for advanced biomedical imaging applications.