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In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane
Published on: November 28, 2019
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Comprehensive quantitative characterization of the human term amnion proteome.
Eva Avilla-Royo1,2, Katharina Gegenschatz-Schmid1, Jonas Grossmann3,4
1Department of Obstetrics, University and University Hospital of Zurich, 8091 Zurich, Switzerland.
Matrix Biology Plus
|November 12, 2021
Summary
Researchers characterized the healthy human term amnion proteome, identifying over 5000 proteins. This comprehensive dataset advances understanding of fetal membrane biology and aids development of healing biomaterials.
Area of Science:
- Proteomics
- Reproductive Biology
- Biomaterials Science
Background:
- Fetal membrane (FM) integrity is crucial for pregnancy; loss can lead to adverse outcomes.
- Lack of biomaterials for FM sealing and healing stems from incomplete knowledge of FM biology.
- A comprehensive proteomic description of the human amnion is currently unavailable.
Purpose of the Study:
- To comprehensively characterize the healthy human term amnion proteome.
- To provide a foundation for understanding fetal membrane complications.
- To facilitate the development of novel healing-inducing biomaterials for fetal membranes.
Main Methods:
- Optimized protein sample preparation and offline fractionation.
- Liquid chromatography coupled to mass spectrometry (LC-MS) analysis.
- Proteomic dataset deposited in ProteomeXchange Consortium (identifier PXD019410).
Main Results:
- Characterization of the healthy human term amnion proteome.
- Identification of over 5000 proteins, significantly exceeding previous reports.
- Coverage of over 40% of previously reported transcripts in RNA sequencing datasets.
Conclusions:
- This study presents the most comprehensive human amnion proteome to date.
- The findings provide a basis for studying compromised and preterm ruptured FMs.
- The proteomic data serves as a stepping-stone for developing innovative healing biomaterials for fetal membranes.
