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Delivery of CRISPR-Cas9 system for screening and editing RNA binding proteins in cancer
Jingyue Yan1, Diana D Kang1, Gillian Turnbull1
1Division of Pharmaceutics & Pharmacology, College of Pharmacy, The Ohio State University, Columbus, OH 43210, United States.
Abstract:
RNA-binding proteins (RBPs) play an important role in RNA metabolism, regulating the stability, localization, and functional dynamics of RNAs. Alternation in the RBP-RNA network has profound implications in cellular physiology, and is related to the development and spread of cancer in certain cases. To regulate the expression of specific genes and their biological activities, various strategies have been applied to target RBPs for cancer treatments, including small-molecule inhibitors, small-interfering RNA, peptides, and aptamers. Recently, the deployment of the CRISPR-Cas9 technology has provided a new platform for RBP screening and regulation. This review summarizes the delivery systems of the CRISPR-Cas9 system and their role in RBP-based cancer therapeutics, including identification of novel RBPs and regulation of cancer-associated RBPs. The efficient delivery of the CRISPR-Cas9 system is important to the profound understanding and clinical transition of RBPs as cancer therapeutic targets.
Insights
RNA-binding proteins (RBPs) are crucial in RNA metabolism and cancer. CRISPR-Cas9 technology offers new ways to target RBPs for cancer therapy, improving drug discovery and treatment strategies.
Area of Science:
- Molecular Biology
- Genetics
- Cancer Research
Background:
- RNA-binding proteins (RBPs) regulate RNA stability, localization, and dynamics.
- Dysregulation of the RBP-RNA network is implicated in cancer development and progression.
- Current therapeutic strategies targeting RBPs include small molecules, RNA interference, peptides, and aptamers.
Purpose of the Study:
- To review the role of CRISPR-Cas9 technology in RBP-based cancer therapeutics.
- To summarize CRISPR-Cas9 delivery systems for RBP screening and regulation.
- To highlight the potential of RBPs as therapeutic targets in oncology.
Main Methods:
- Review of current literature on CRISPR-Cas9 delivery systems.
- Analysis of studies employing CRISPR-Cas9 for RBP identification and functional characterization.
- Examination of RBP-targeting strategies in cancer treatment.
Main Results:
- CRISPR-Cas9 provides a novel platform for high-throughput screening of RBPs.
- CRISPR-Cas9 enables precise regulation of cancer-associated RBPs.
- Efficient delivery of CRISPR-Cas9 systems is critical for RBP-based therapeutic development.
Conclusions:
- CRISPR-Cas9 technology significantly advances the identification and functional study of RBPs in cancer.
- Targeting RBPs with CRISPR-Cas9 offers a promising avenue for novel cancer therapeutics.
- Optimizing CRISPR-Cas9 delivery is essential for clinical translation of RBP-targeted cancer therapies.
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