Related Experiment Video

Updated: Oct 13, 2025

Phage-mediated Delivery of Targeted sRNA Constructs to Knock Down Gene Expression in E. coli
08:25

Phage-mediated Delivery of Targeted sRNA Constructs to Knock Down Gene Expression in E. coli

Published on: March 20, 2016

12.8K

Bacteriophages and phage-delivered CRISPR-Cas system as antibacterial therapy

Ting-Kuang Yeh1, Shio-Shin Jean2, Yu-Lin Lee3

  • 1Division of Infectious Diseases, Department of Internal Medicine, Taichung Veterans General Hospital, Taichung, Taiwan.

International Journal of Antimicrobial Agents
|November 12, 2021
PubMed

Insights

Multidrug-resistant bacterial infections are a growing global threat. This review explores bacteriophage therapy and CRISPR-Cas antimicrobials, highlighting phage-delivered CRISPR-Cas systems as a promising solution.

Area of Science:

  • Microbiology
  • Genetics
  • Infectious Diseases

Background:

  • Increasing global prevalence of multidrug-resistant (MDR) bacterial infections.
  • Antimicrobial resistance (AMR) poses a significant threat to public health.
  • Bacteriophage therapy and CRISPR-Cas systems show potential as novel antimicrobial strategies.

Purpose of the Study:

  • To review randomized controlled trials on bacteriophage therapy.
  • To elucidate the mechanisms of CRISPR-Cas system antimicrobials.
  • To consolidate research on phage-delivered CRISPR-Cas system antimicrobials.

Main Methods:

  • Literature review of randomized controlled trials on bacteriophage therapy.
  • Integration of CRISPR-Cas antimicrobial mechanisms into a schematic diagram.
  • Consolidation of research on phage-based delivery of CRISPR-Cas systems.

Main Results:

  • Bacteriophage therapy has demonstrated successful applications against MDR bacteria.
  • CRISPR-Cas systems offer bactericidal effects in vivo.
  • Phage-based vectors are an effective method for delivering CRISPR-Cas systems into target bacteria.

Conclusions:

  • Phage therapy and CRISPR-Cas antimicrobials represent promising avenues for combating MDR infections.
  • Phage-delivered CRISPR-Cas systems offer a targeted and effective approach to antimicrobial treatment.
  • Further research and clinical trials are warranted to fully realize the potential of these technologies.
Keywords:
AntimicrobialBacteriophage therapyCRISPR-CasPhage-based vector

More Related Videos

Application of CRISPR Interference CRISPRi for Gene Silencing in Pathogenic Species of Leptospira14:49

Application of CRISPR Interference CRISPRi for Gene Silencing in Pathogenic Species of Leptospira

Published on: August 14, 2021

5.3K
Substrate Generation for Endonucleases of CRISPR/Cas Systems11:53

Substrate Generation for Endonucleases of CRISPR/Cas Systems

Published on: September 8, 2012

27.6K

Related Concept Videos

CRISPR and crRNAs02:53

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.8K
The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
258
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
615
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
53.4K
Lytic Cycle of Bacteriophages01:30

Lytic Cycle of Bacteriophages

Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...
73.0K
DNA Bacteriophages01:26

DNA Bacteriophages

Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
254