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Updated: Oct 13, 2025

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Platelet-Rich Fibrin Decreases the Inflammatory Response of Mesenchymal Cells

Zahra Kargarpour1, Jila Nasirzade1, Layla Panahipour1

  • 1Department of Oral Biology, Medical University of Vienna, 1090 Vienna, Austria.

International Journal of Molecular Sciences
|November 13, 2021
PubMed

Insights

Platelet-rich fibrin (PRF) and its components effectively reduce inflammatory responses in various murine mesenchymal cells. This study demonstrates PRF

Area of Science:

  • Regenerative Medicine
  • Immunology
  • Cell Biology

Background:

  • Chronic inflammation involves mesenchymal cells producing inflammatory mediators.
  • Platelet-rich fibrin (PRF) shows anti-inflammatory effects in macrophages, but its impact on mesenchymal cells is unknown.

Purpose of the Study:

  • To investigate the anti-inflammatory effects of platelet-rich fibrin (PRF) and its blood-derived components on mesenchymal cells.

Main Methods:

  • Mesenchymal cells (ST2, 3T3-L1, murine calvarial cells) were exposed to inflammatory cytokines and lysates from platelet-poor plasma (PPP), buffy coat (BC), and PRF.
  • Western blotting and nuclear translocation assays assessed inflammatory signaling pathways (p65 phosphorylation).
  • Expression of inflammatory mediators (IL6, iNOS, CCL5) was quantified.

Main Results:

  • Lysates of PPP, BC, and PRF significantly reduced cytokine-induced IL6 and iNOS expression in ST2 cells.
  • These components also decreased p65 phosphorylation and nuclear translocation in ST2 cells.
  • PPP, BC, and PRF diminished inflammatory mediators in 3T3-L1 and murine calvarial cells, but not in human gingival fibroblasts or HSC2 cells.

Conclusions:

  • Both liquid and solid forms of PRF exhibit potent anti-inflammatory activity in murine mesenchymal cells.
  • The anti-inflammatory effects are mediated through the suppression of key inflammatory signaling pathways.
  • PRF components may hold therapeutic potential for inflammatory conditions involving mesenchymal cells.
Keywords:
Toll-like receptorcytokineinflammationmesenchymal cellsplatelet-rich fibrin

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