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Related Concept Videos

T Cell Types and Functions01:24

T Cell Types and Functions

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When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
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Related Experiment Video

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Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes
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C-Reactive Protein Controls IL-23 Production by Human Monocytes.

Chiara E Geyer1, Melissa Newling2,3, Lathees Sritharan2,3

  • 1Center for Experimental and Molecular Medicine, Amsterdam Infection & Immunity Institute, Amsterdam UMC, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands.

International Journal of Molecular Sciences
|November 13, 2021
PubMed
Summary
This summary is machine-generated.

C-reactive protein (CRP) uniquely induces the pro-inflammatory cytokine IL-23 in human monocytes. This finding highlights CRP's distinct role in inflammation beyond its function as a marker.

Keywords:
C-reactive proteinFc receptorIL-23Sepsisinflammationinflammatory bowel diseasemonocyte

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Area of Science:

  • Immunology
  • Molecular Biology

Background:

  • C-reactive protein (CRP) is an acute-phase protein elevated during inflammation.
  • While CRP is a marker of inflammation, it can also actively promote it.
  • Human monocytes are key innate immune cells involved in inflammatory responses.

Purpose of the Study:

  • To investigate the cytokine profile induced by CRP in human monocytes.
  • To compare CRP's effect on cytokine production with pattern recognition receptor (PRR) ligands.
  • To elucidate the transcriptional mechanisms underlying CRP-induced cytokine production.

Main Methods:

  • Stimulation of human monocytes with CRP and various PRR ligands.
  • Analysis of cytokine production, specifically IL-23.
  • Measurement of gene transcription for IL-23 and IL-12 family members.

Main Results:

  • CRP uniquely induced the pro-inflammatory cytokine IL-23 production by monocytes.
  • CRP primarily enhanced IL23A gene transcription, unlike PRR ligands.
  • Combined CRP and PRR ligand stimulation significantly reduced IL-23 production.

Conclusions:

  • CRP acts as a distinct ligand that promotes IL-23 production in monocytes.
  • This CRP-mediated IL-23 induction may influence systemic immune responses in inflammatory conditions.
  • Understanding CRP's role in IL-23 production offers insights into immune regulation during infection and inflammation.