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Updated: Oct 13, 2025

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
Published on: March 30, 2019
MiR-497-5p down-regulates CDCA4 to restrains lung squamous cell carcinoma progression
Jiangwei Hu1, Xinqin Xiang1, Wei Guan1
1Department of Cardiovascular Surgery, Yiwu Central Hospital, No.699 Jiangdong Dong Lu, Yiwu City, 322000, Zhejiang Province, China.
Background:
So far, few have concerned miR-497-5p in lung squamous cell carcinoma (LUSC).
Methods:
MiR-497-5p expression in LUSC was measured by qRT-PCR. Its impacts on tumor-related cell behaviors were investigated by CCK8 assay, scratch healing assay, flow cytometry and Transwell invasion methods. In addition, interaction between miR-497-5p and CDCA4 in LUSC was also elucidated through rescue experiment, western blot, dual-luciferase, and bioinformatics analysis.
Results:
Low level of miR-497-5p was confirmed in LUSC tissue and cells. Overexpressed miR-497-5p markedly inhibited cancer progression. miR-497-5p restrained CDCA4 expression. Rescue assay showed that overexpressing miR-497-5p eliminated effect of overexpressed CDCA4.
Conclusion:
By targeting CDCA4, miR-497-5p restrained development of LUSC.
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