Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Bone marrow matrix modulation of HL-60 phenotype.

S D Luikart1, J L Sackrison, C A Maniglia

  • 1Department of Medicine/Division of Oncology, University of Minnesota Medical School, Minneapolis.

Blood
|October 1, 1987
PubMed
Summary

The bone marrow extracellular matrix significantly influences leukemic cell differentiation, altering proliferation, morphology, and TPA sensitivity. This matrix, not conditioned media, impacts HL-60 cell behavior in vitro.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluation of 25 OH Vitamin D in chronic renal failure and end stage renal disease subjects.

The Journal of steroid biochemistry and molecular biology·2004
Same author

Hypovitaminosis D in a normal, apparently healthy urban European population.

The Journal of steroid biochemistry and molecular biology·2004
Same author

Long-term blood product transfusion support for patients with myelodysplastic syndromes (MDS): cost analysis and complications.

Leukemia research·1999
Same author

Phase I trial of etoposide, carboplatin, and GM-CSF in extensive small-cell lung cancer: a Cancer and Leukemia Group B study (CALGB 8832).

American journal of clinical oncology·1997
Same author

A phase I/II trial of etoposide and cisplatin in extensive small cell lung cancer: a cancer and leukemia group B study.

Lung cancer (Amsterdam, Netherlands)·1996
Same author

Antibody immunoglobulin G (IgG) against human prostatic specific antigen (PSA) as a carrier protein for chemotherapeutic drugs to human prostate tumors: Part 1. A double immunofluorescence analysis.

The Anatomical record·1996

Area of Science:

  • Biomedical Science
  • Cell Biology
  • Cancer Research

Background:

  • Cellular differentiation is regulated by the microenvironment.
  • Leukemic cell behavior can be influenced by bone marrow stromal cells.

Purpose of the Study:

  • To investigate the impact of bone marrow stroma on leukemic cell differentiation.
  • To determine if the extracellular matrix or conditioned media from stromal cells affects HL-60 cell phenotype.

Main Methods:

  • HL-60 human promyelocytic leukemia cells were cultured with Triton-treated extracellular matrix from normal human bone marrow stromal cells.
  • Phenotypic changes in HL-60 cells were assessed, including proliferation, morphology, esterase activity, Fc rosette formation, and TPA sensitivity.

Main Results:

  • The bone marrow matrix microenvironment significantly altered HL-60 cell phenotype.
  • HL-60 proliferation, morphology, esterase activity, Fc rosette formation, and TPA sensitivity were all impacted by matrix molecules.
  • Stromal cell-conditioned media did not alter HL-60 phenotype.

Conclusions:

  • HL-60 cells retain responsiveness to the bone marrow stromal cell-derived matrix despite autonomous growth.
  • The extracellular matrix plays a crucial role in regulating leukemic cell behavior.
  • In vitro studies of leukemic cell-marrow stroma interactions offer insights into leukemic cell regulation.

Related Experiment Videos