Micelle to cyclodextrin stacking in open-tubular liquid chromatography using capillaries coated with surfactant...

In-line sample concentration by micelle to cyclodextrin stacking (MCDS) in open-tubular liquid chromatography (OT-LC) with UV detection is described. OT-LC of two sets of analytes (small-molecule drugs and neutral alkenylbenzenes) was by the use of a fused-silica capillary that was coated with admicelles of didodecyldimethyl ammonium bromide (DDAB). These admicelles acted as a stationary chromatographic pseudophase. The mobile phase was 25 mM sodium tetraborate in 10% methanol, pH 9.2. MCDS was by long pressure injection of samples prepared in 10 mM hexadecyltrimethyl ammonium bromide (CTAB) in 25 mM sodium tetraborate, pH 9.2 (buffer), followed by injection of 50 mM α-CD in buffer (CD solution). Stacking was by application of voltage at -20 kV prior to pressure-driven OT-LC. The factors that influenced MCDS such as type and concentration of CD, concentration of CTAB in the sample, injection time ratio of the sample and the CD solution and stacking time were studied. Under optimised conditions, sensitivity enhancement factors (SEFs) were between 19 and 23, linear ranges were between 0.5 and 10 µg/mL with r² > 0.99 and inter-day/intra-day repeatability in retention time and peak area were ≤5.6% for the model small-molecule drugs. Application to real samples was by the determination of potentially toxic alkenylbenzenes (SEFs = 10 to 12) in basil-leaf and whole-clove extracts. The assay results were comparable to those obtained from an in-house high-performance liquid chromatography-UV method.