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Effect of curcumin on sperm parameters after the cryopreservation
Fatma Nur Karakus1, Sibel Bulgurcuoglu Kuran2, Seyhun Solakoglu3
1Medical Doctor, Cengiz Gökçek Women's Health and Children Hospital, Department of Histology and Embryology, Gaziantep,Turkey.
European Journal of Obstetrics, Gynecology, and Reproductive Biology
|November 13, 2021
Summary
Curcumin supplementation during human sperm cryopreservation significantly improves post-thaw sperm quality. This antioxidant enhances motility, chromatin condensation, and DNA integrity, mitigating cryodamage for fertility preservation.
Area of Science:
- Reproductive Biology
- Cryobiology
- Biochemistry
Background:
- Cryopreservation is crucial for assisted reproduction and male fertility preservation.
- Cryopreservation significantly impairs human sperm quality.
- Curcumin exhibits known antioxidant properties beneficial for various cells.
Purpose of the Study:
- To investigate the impact of curcumin on human sperm quality post-cryopreservation.
- To evaluate changes in sperm viability, morphology, motility, chromatin condensation, and DNA integrity.
Main Methods:
- Semen from 23 normozoospermic men was divided into control, DMSO, and curcumin (10 µM) groups.
- Sperm parameters (viability, morphology, motility, chromatin packaging, DNA integrity) were assessed pre- and post-thaw.
- Samples were analyzed using specific staining and microscopy techniques.
Main Results:
- Cryopreservation markedly reduced sperm parameters across all groups (P < 0.001).
- The curcumin group showed significant improvements in progressive motility, chromatin condensation, and DNA integrity post-thaw (P < 0.001) compared to control and DMSO groups.
Conclusions:
- Curcumin addition to cryopreservation medium enhances post-thaw human sperm quality.
- Curcumin effectively mitigates the negative effects of cryopreservation on spermatozoa.
- Further research into antioxidants like curcumin for sperm cryopreservation is warranted.
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