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Updated: Oct 13, 2025

Microfluidic Bioprinting for Engineering Vascularized Tissues and Organoids

Microfluidic Bioprinting for Engineering Vascularized Tissues and Organoids

Published on: August 11, 2017

A strategy to engineer vascularized tissue constructs by optimizing and maintaining the geometry.

Yi-Jung Hsu¹, Shih-Yen Wei¹, Teng-Yen Lin¹

¹Department of Materials Science and Engineering, National Tsing-Hua University, Hsinchu, Taiwan.

Acta Biomaterialia

|November 14, 2021

Summary

This summary is machine-generated.

Engineered hexagonal hydrogels rapidly form large, functional vascular networks in normal and diabetic mice, improving islet cell survival and insulin secretion for regenerative therapies.

Keywords:

Cell-laden hydrogel structures Diffusion-based computational simulations Vascularized tissues

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Last Updated: Oct 13, 2025

Microfluidic Bioprinting for Engineering Vascularized Tissues and Organoids

Microfluidic Bioprinting for Engineering Vascularized Tissues and Organoids

Published on: August 11, 2017

Stepwise Cell Seeding on Tessellated Scaffolds to Study Sprouting Blood Vessels

Stepwise Cell Seeding on Tessellated Scaffolds to Study Sprouting Blood Vessels

Published on: January 14, 2021

Generation and Grafting of Tissue-engineered Vessels in a Mouse Model

Generation and Grafting of Tissue-engineered Vessels in a Mouse Model

Published on: March 18, 2015

Area of Science:

Biomaterials Science
Regenerative Medicine
Vascular Biology

Background:

Engineered tissue success relies on rapid vascularization, which is hindered in diabetic conditions.
Current hydrogels face limitations in supporting thick, vascularized tissue due to decreased transport diffusivity.
Optimizing hydrogel geometry is crucial for overcoming diffusion limitations and enabling thicker constructs.

Purpose of the Study:

To computationally optimize hydrogel structure for enhanced diffusion and vascular network formation.
To develop a cell-based strategy for creating large, thick, and functional vascularized tissue constructs.
To evaluate the efficacy of these constructs in supporting transplanted islet cells, particularly in a diabetic environment.

Main Methods:

Utilized diffusion-based computational simulations to determine optimal hydrogel geometries.
Engineered hexagonal, cell-laden hydrogel structures with a protective spacer for enhanced stability.
Implanted hydrogel constructs in both normal and diabetic mouse models.

Main Results:

Hexagonal hydrogel structures significantly improved diffusion, supporting cell viability and spreading.
Generated large (∼17 mm diameter, ∼1.5 mm thickness) and thick perfused vascular networks within 7 days.
Demonstrated enhanced islet cell viability and insulin secretion upon subcutaneous transplantation into the engineered vascular bed.
Achieved functional vascularization in both normal and diabetic mice.

Conclusions:

Developed a promising strategy for rapidly generating large, thick, functional vascularized tissue constructs using optimized hexagonal hydrogels.
The engineered vascular beds effectively supported transplanted islet cells, showing potential for regenerative therapy.
The approach offers a viable solution for vascularizing engineered tissues, addressing limitations in diabetic conditions and paving the way for other tissue engineering applications.