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Updated: Aug 5, 2026

Colorimetric Paper-based Detection of Escherichia coli, Salmonella spp., and Listeria monocytogenes from Large Volumes of Agricultural Water
Published on: June 9, 2014
A multistep enrichment process with custom growth medium improves resuscitation of chlorine-stressed coliforms from
Jennifer M Mobberley1, Scott K Cooley2, Mark W Widder3
1Chemical and Biological Signatures Group, National Security Directorate, Pacific Northwest National Laboratory, P.O. Box 999, Richland, WA 99352, USA.
Abstract:
Resuscitation and detection of stressed total coliforms in chlorinated water samples is needed to assess and prevent health effects from adverse exposure. In this study, we report that the addition of a growth enhancer mix consisting of trehalose, sodium pyruvate, magnesium chloride, and 1× trace mineral supplement improved growth of microorganisms from chlorinated secondary effluent in the base medium with Colilert-18. Improving growth of chlorine stressed microorganisms from secondary effluent is crucial to decreased detection time from 18 to 8 h.
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