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Inhibition of myogenic differentiation by fibroblast growth factor or type beta transforming growth factor does not

G Spizz1, J S Hu, E N Olson

  • 1Department of Biochemistry and Molecular Biology, University of Texas, M. D. Anderson Hospital and Tumor Institute, Houston 77030.

Developmental Biology
|October 1, 1987
PubMed

Insights

Persistent c-myc expression is not required for growth factor inhibition of skeletal muscle differentiation. Growth factors like FGF and TGF-beta can suppress myogenesis, even when c-myc levels remain low.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Skeletal muscle differentiation involves MCK mRNA accumulation.
  • Myogenesis can be inhibited by serum, FGF, and TGF-beta.
  • The role of c-myc in this inhibition is not fully understood.

Purpose of the Study:

  • To investigate the involvement of c-myc in growth factor-dependent inhibition of myogenesis.
  • To examine the relationship between c-myc expression and myogenic differentiation in BC3H1 cells.

Main Methods:

  • Utilized the BC3H1 nonfusing myogenic cell line.
  • Manipulated serum concentration and exposure to FGF and TGF-beta.
  • Monitored c-myc and MCK mRNA expression levels.

Main Results:

  • Withdrawal from the cell cycle led to decreased c-myc mRNA and increased MCK mRNA.
  • TGF-beta suppressed MCK mRNA despite c-myc decline.
  • FGF and TGF-beta induced transient c-myc expression but inhibited muscle-specific gene products.

Conclusions:

  • Persistent c-myc expression is not essential for growth factor-mediated inhibition of myogenic differentiation.
  • Growth factors can inhibit myogenesis independently of sustained c-myc activity.

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